Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP, adenosine and thrombin) based on DNA/Ag nanoclusters fluorescence light-up system
Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP, adenosine and thrombin) based on DNA/Ag nanoclusters fluorescence light-up system
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基于DNA/Ag纳米团簇荧光发光系统的超灵敏通用荧光适体传感器用于检测生物分子（ATP、腺苷和凝血酶）
DOI：
10.1016/j.bios.2015.12.015
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发表时间：
2016-05-15
影响因子：
12.6
通讯作者：
Yao, Tian-ming
中科院分区：
文献类型：
--
作者：
Zhu, Ying;Hu, Xiao-chun;Yao, Tian-ming
我们在这里报告了一个超灵敏的策略的基础上，在接近银纳米团簇的核酸适体和荧光开启能力的鸟嘌呤（G-丰富）的DNA序列的构象变化的检测三磷酸腺苷（ATP），腺苷（A）和凝血酶（TB）。在此，我们设计了两个定制的DNA序列，分别标记为互补DNA（缩写为c-DNA）和信号探针DNA（缩写为s-DNA）。c-DNA被设计为一种特殊结构，由3 '端与适体互补的序列和5'端富含鸟嘌呤的DNA序列组成; s-DNA在3 '端含有负责Ag NC模板合成的富含胞嘧啶的序列，在5'端含有与部分c-DNA互补的连接序列（适体的一部分）。在靶标存在的情况下，适体与靶标缔合，导致双链体DNA（dsDNA）的形成，DNA-Ag NCs此后可以接近富含鸟嘌呤的序列，导致增强的荧光信号读出。该传感系统在三种生物分子的检测中取得了成功，并得到了广泛的应用。ATP、腺苷和凝血酶的线性范围分别为0.5-8.0 μ M、0.5-7.0 μ M和50-900 nM，检测限分别为91.6 nM、103.4 nM和8.4 nM。这种无标记和开启的荧光传感系统采用这里提出的机制，结果是敏感的，选择性的，方便的检测生物分子，而无需洗涤和分离步骤。(C)2015 Elsevier B. V.版权所有。
We report here an ultrasensitive strategy based on the recognition-induced conformational alteration of aptamer and fluorescence turn-on abilities of guanine-rich (G-rich) DNA sequence in proximity to silver nanoclusters for adenosine triphosphate (ATP), adenosine (A) and thrombin (TB) detection. Herein, we designed two tailored DNA sequences noted as complementary DNA (abbreviated as c-DNA) and signal probe DNA (abbreviated as s-DNA), respectively. c-DNA is designed as a special structure consisting of a sequence complementary to aptamer at the 3'-end and a guanine-rich DNA sequence at the 5'-end; s-DNA contains a cytosine-rich sequence responsible for Ag NCs templated synthesis at the 3'-end and a link sequence (part of aptamer) complementary to partial of the c-DNA at the 5'-end. In the presence of target, the aptamer associated with the target, resulting in the formation of duplex DNA (dsDNA), the DNA-Ag NCs thereafter could close to the guanine-rich sequence, leading to enhanced fluorescence signal readout. The widespread application of the sensing system is achieved success in the detection of three biomolecules. ATP, adenosine and thrombin in the range of 0.5-8.0 mu M, 0.5-7.0 mu M and 50-900 nM could be linearly detected with the detection limits of 91.6 nM, 103.4 nM and 8.4 nM, respectively. This label-free and turn-on fluorescent sensing system employing the mechanism proposed here turns out to be sensitive, selective, and convenient for the detection of biomolecules without washing and separation steps. (C) 2015 Elsevier B.V. All rights reserved.