Polyethylene and methyl methacrylate particle-stimulated inflammatory tissue and macrophages up-regulate bone resorption in a murine neonatal calvaria in vitro organ system
Polyethylene and methyl methacrylate particle-stimulated inflammatory tissue and macrophages up-regulate bone resorption in a murine neonatal calvaria in vitro organ system
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DOI：
10.1016/s0736-0266(02)00019-0
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发表时间：
2002-09-01
影响因子：
2.8
通讯作者：
Wooley, PH
中科院分区：
文献类型：
--
作者：
Ren, WP;Wu, B;Wooley, PH
有相当多的证据表明，骨科磨损碎片在关节假体无菌性松动的病理中起着至关重要的作用。本研究采用小鼠气袋模型，研究了甲基丙烯酸甲酯和聚乙烯刺激炎症膜对骨吸收的影响。我们还研究了暴露于聚合物颗粒的RAW 264.7小鼠巨噬细胞产生影响骨代谢因子的能力。新生儿颅骨与活化巨噬细胞的囊膜或条件培养基共培养。通过培养骨中钙的释放量来测定骨吸收，并测定骨切片和培养基中抗酒石酸酸性磷酸酶的活性。结果表明，经甲基丙烯酸甲酯和聚乙烯活化的炎性袋膜增强破骨细胞骨吸收。来自颗粒刺激小鼠巨噬细胞的条件培养基也刺激了骨吸收，尽管这种作用比炎性袋膜诱导的吸收弱。将颗粒直接添加到培养的颅骨培养基中，对骨吸收的影响不大。我们的观察表明，炎症组织和颗粒引起的巨噬细胞都可以刺激培养的小鼠新生颅骨骨的骨吸收。这个简单的体外骨吸收系统使我们能够研究磨损碎片诱导骨吸收的基本细胞和分子机制，并筛选无菌性松动的潜在治疗方法。(C) 2002骨科研究学会。Elsevier Science Ltd.出版。版权所有。
There is considerable evidence that orthopaedic wear debris plays a crucial role in the pathology of aseptic loosening of joint prostheses. This study examined the effect of inflammatory membranes stimulated with methyl methacrylate and polyethylene on bone resorption, using the murine air pouch model. The capacity of RAW 264.7 mouse macrophages exposed to polymer particles to produce factors affecting bone metabolism was also studied. Neonatal calvaria bones were co-cultured with either pouch membranes or conditioned media from activated macrophages. Bone resorption was measured by the release of calcium from cultured bones, and the activity of tartrate-resistant acid phosphatase in both bone sections and culture medium was also assayed. Results showed that inflammatory Pouch membrane activated by methyl methacrylate and polyethylene enhanced osteoclastic bone resorption. Conditioned media from particles Stimulated mouse macrophages also stimulated bone resorption, although this effect was weaker than resorption induced by inflammatory Pouch membranes. The addition of the particles directly into the medium of Cultured calvaria bones had little effect on bone resorption. Our observations indicate that both inflammatory tissue and macrophages provoked by particles can stimulate bone resorption in Cultured mouse neonatal calvaria bones. This simple in vitro bone resorption system allows us to investigate the fundamental cellular and molecular mechanism of wear debris induced bone resorption and to screen potential therapeutic approaches for aseptic loosening. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.