CLIP-continuous live imaging platform for direct observation of C. elegans physiological processes
CLIP-continuous live imaging platform for direct observation of C. elegans physiological processes
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DOI：
10.1039/c3lc50300c
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Lu, Hang
中科院分区：
文献类型：
--
作者：
Krajniak, Jan;Hao, Yan;Lu, Hang
随着时间的推移，直接观察某些模式生物的发育和生理变化在技术上具有挑战性。在模式生物秀丽隐杆线虫中，这些研究需要在生理良性条件下频繁或连续成像。然而，标准方法使用麻醉剂，胶水或微珠，这会阻止动物在实验期间进食。因此，随着时间的推移，动物的正常生理功能可能会受到影响。在这里，我们提出了一个平台设计的动态研究C。优雅的该系统能够在良性条件下仅固定动物的身体，而不会发生物理变形。同时，动物的头部在实验期间保持自由移动和进食。这允许固定和喂养动物的高分辨率和高放大倍数的荧光成像。该系统非常容易制造，设置和操作，并应广泛适用于发育和生理研究中的许多问题。
Direct observation of developmental and physiological changes in certain model organisms over time has been technically challenging. In the model organism Caenorhabditis elegans, these studies require frequent or continuous imaging at physiologically benign conditions. However, standard methods use anaesthetics, glue, or microbeads, which prevent animals from feeding during the experiment. Thus, the animals' normal physiological function may be affected over time. Here we present a platform designed for dynamic studies of C. elegans. The system is capable of immobilizing only the animals' bodies under benign conditions and without physical deformation. Simultaneously, the animals' heads remain free to move and feed for the duration of the experiment. This allows for high-resolution and high-magnification fluorescent imaging of immobilized and feeding animals. The system is very easy to fabricate, set up, and operate, and should be widely applicable to many problems in developmental and physiological studies.