Inheritance of steroid-independent male sexual behavior in male offspring of B6D2F1 mice.
Inheritance of steroid-independent male sexual behavior in male offspring of B6D2F1 mice.
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DOI：
10.1016/j.yhbeh.2016.02.003
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发表时间：
2016-04
影响因子：
3.5
通讯作者：
Park JH
中科院分区：
文献类型：
--
作者：
McInnis CM;Bonthuis PJ;Rissman EF;Park JH
性腺类固醇激素在调节男性性行为中的重要性已得到公认。男性性行为的个体差异，独立于性腺类固醇，是普遍存在于广泛的物种，包括man. However，类固醇独立的男性性行为的遗传机制知之甚少。高比例的B6 D2 F1杂交雄性小鼠表现出类固醇非依赖性雄性性行为（确定为“maters”），提供了一种小鼠模型，开辟了研究在性腺激素缺乏的情况下调节雄性性行为的机制的途径。最近的研究揭示了几种蛋白质在调节B6 D2 F1雄性小鼠的类固醇非依赖性雄性性行为中起重要作用，包括淀粉样前体蛋白（APP），tau和突触素。我们的研究的具体目标是确定类固醇非依赖性男性性行为是否是一种遗传性状，通过确定它是否依赖于行为表型的B6 D2 F1公鹿，以及APP，tau蛋白和突触素的差异表达在内侧视前区发现的B6 D2 F1公鹿，没有交配后性腺切除术是相似的，在他们的男性后代。成年B6 D2 F1雄性小鼠与C57 BL/6 J雌性小鼠交配后，将它们及其雄性后代（BXB 1）切除睾丸，并鉴定为“母本”或“非母本”。一个显着的比例的BXB 1的母鼠只从B6 D2 F1的母鼠，表明类固醇非依赖性的雄性性行为的行为表型的B6 D2 F1杂交雄性，当与C57 BL/6 J雌性小鼠杂交，是由他们的雄性后代遗传。此外，APP，tau蛋白，和突触素升高，在内侧视前区的B6 D2 F1和BXB 1母相对于B6 D2 F1和BXB 1非母，分别，这表明一个潜在的遗传机制的类固醇非依赖性男性性行为的遗传。
The importance of gonadal steroids in modulating male sexual behavior is well established. Individual differences in male sexual behavior, independent of gonadal steroids, are prevalent across a wide range of species, including man. However, the genetic mechanisms underlying steroid-independent male sexual behavior are poorly understood. A high proportion of B6D2F1 hybrid male mice demonstrate steroid-independent male sexual behavior (identified as “maters”), providing a mouse model that opens up avenues of investigation into the mechanisms regulating male sexual behavior in the absence of gonadal hormones. Recent studies have revealed several proteins that play a significant factor in regulating steroid-independent male sexual behavior in B6D2F1 male mice, including amyloid precursor protein (APP), tau, and synaptophysin. The specific goals of our study were to determine whether steroid-independent male sexual behavior was a heritable trait by determining if it was dependent upon the behavioral phenotype of the B6D2F1 sire, and whether the differential expression of APP, tau, and synaptophysin in the medial preoptic area found in the B6D2F1 sires that did and did not mate after gonadectomy was similar to those found in their male offspring. After adult B6D2F1 male mice were bred with C57BL/6J female mice, they and their male offspring (BXB1) were orchidectomized and identified as either maters or “non-maters.” A significant proportion of the BXB1 maters were sired only from B6D2F1 maters, indicating that the steroid-independent male sexual behavior behavioral phenotype of the B6D2F1 hybrid males, when crossed with C57BL/6J female mice, is inherited by their male offspring. Additionally, APP, tau, and synaptophysin were elevated in in the medial preoptic area in both the B6D2F1 and BXB1 maters relative to the B6D2F1 and BXB1 non-maters, respectively, suggesting a potential genetic mechanism for the inheritance of steroid-independent male sexual behavior.