The endocytic machinery in nerve terminals surrounds sites of exocytosis
The endocytic machinery in nerve terminals surrounds sites of exocytosis
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DOI：
10.1016/s0960-9822(00)80087-1
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发表时间：
1999-12-02
期刊：
影响因子：
9.2
通讯作者：
Kelly, RB
中科院分区：
文献类型：
--
作者：
Roos, J;Kelly, RB
在大多数内吞作用模型中，内吞机制通过待内化的质膜蛋白质的胞质尾部从细胞质中募集。在突触中，突触囊泡再循环所需的内吞机制定位于膜相关的“热点”，这似乎不是真的[1，2]。在果蝇神经肌肉接头中，多结构域蛋白Dap160也定位于热点[3]，并具有锚定蛋白的一些预期特征。将内吞机制锚定到质膜可能有助于突触囊泡回收的显着速度[4]。在这里，我们报告说，内吞机制周围的网站被认为是网站的胞吐作用。我们建议，径向分布的突触囊泡回收机械已经存在于质膜上的未受刺激的神经末梢是一个基本单位的突触前组织，并允许神经末梢提取最大的回收效率的常规内吞机械。
In most models of endocytosis, the endocytic machinery is recruited from the cytoplasm by cytoplasmic tails of the plasma membrane proteins that are to be internalized. This does not appear to be true at synapses where the endocytic machinery required for synaptic vesicle recycling is localized to membrane-associated 'hot spots' [1,2]. In Drosophila neuromuscular junctions, the multi-domain protein Dap160 is also localized to hot spots [3] and has some characteristics expected of an anchoring protein. Anchoring the endocytic machinery to the plasma membrane might help contribute to the remarkable speed of synaptic vesicle recycling [4]. Here, we report that the endocytic machinery surrounds sites that are believed to be sites of exocytosis. We propose that the radial distribution of the synaptic vesicle recycling machinery already present on the plasma membrane in unstimulated nerve terminals is a fundamental unit of pre-synaptic organization and allows the nerve terminal to extract maximum recycling efficiency out of conventional endocytic machinery.