Tensile properties of contractile and synthetic vascular smooth muscle cells
Tensile properties of contractile and synthetic vascular smooth muscle cells
复制标题
收缩性和合成血管平滑肌细胞的拉伸特性
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
K. Hayashi
中科院分区：
文献类型：
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作者：
H. Miyazaki;Y. Hasegawa;K. Hayashi
采用新开发的张力测试系统测定了合成型和收缩型血管平滑肌细胞（VSMCs）的拉伸性能。采用外植体法和酶消化法分别从兔胸主动脉中分离合成VSMCs和收缩VSMCs。每个细胞漂浮在汉克斯37°C的平衡盐溶液中，用细胞粘合剂将其附着在一对微移液管的细尖端上，然后用线性致动器移动其中一个微移液管，以6 μ m/秒的速度拉伸。用悬臂式测压元件测量施加在测压单元上的载荷；它的伸长是通过视频尺寸分析仪从微移液针尖之间的距离来确定的。在2.4µN和3.4µN的拉伸作用下，合成VSMCs和收缩VSMCs均未发生断裂。其收缩表型的刚度（0.17N/m）显著高于合成表型的刚度（0.09N/m）。合成细胞和收缩细胞的拉伸性能不同是由于细胞骨架结构和收缩装置的不同。
Tensile properties of vascular smooth muscle cells (VSMCs) of synthetic and contractile phenotypes were determined using a newly developed tensile test system. Synthetic and contractile VSMCs were isolated from the rabbit thoracic aorta with an explant and an enzymatic digestion method, respectively. Each cell floated in Hanks' balanced salt solution of 37°C was attached to the fine tips of a pair of micropipettes with a cell adhesive and, then, stretched at the rate of 6µm/sec by moving one of the micropipettes with a linear actuator. Load applied to the cell was measured with a cantilever-type load cell; its elongation was determined from the distance between the micropipette tips using a video dimension analyzer. The synthetic and contractile VSMCs were not broken even at the tensile force of 2.4µN and 3.4µN, respectively. Their stiffness was significantly higher in contractile phenotype (0.17N/m) than in synthetic one (0.09N/m). The different tensile properties between synthetic and contractile cells are attributable to the differences in cytoskeletal structures and contractile apparatus.
影响因子：
33.6
作者：
G. Owens
通讯作者：
G. Owens
影响因子：
20.1
作者：
Reusch, P;Wagdy, H;Ives, HE
通讯作者：
Ives, HE
影响因子：
8.3
作者：
Wang, N
通讯作者：
Wang, N