Biotransformation of 4-chloro-2-nitrophenol into 5-chloro-2-methylbenzoxazole by a marine Bacillus sp strain MW-1
Biotransformation of 4-chloro-2-nitrophenol into 5-chloro-2-methylbenzoxazole by a marine Bacillus sp strain MW-1
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DOI：
10.1007/s10532-011-9512-y
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发表时间：
2012-04-01
期刊：
影响因子：
3.6
通讯作者：
Jain, Rakesh Kumar
中科院分区：
文献类型：
--
作者：
Arora, Pankaj Kumar;Jain, Rakesh Kumar
研究了芽孢杆菌MW-1菌株对4-氯-2-硝基苯酚（4C 2NP）的脱色、解毒和生物转化。该菌株仅在另外的碳源存在下使4C 2NP脱色。通过薄层色谱（TLC）、高效液相色谱（HPLC）和气相色谱-质谱（GC-MS）分析，确定了4-氯-2-氨基苯酚、4-氯-2-乙酰氨基苯酚和5-氯-2-甲基苯并恶唑为代谢产物。静息细胞耗尽4C 2NP，化学计量形成5-氯-2-甲基苯并恶唑。这是第一次报道由任何细菌菌株从4C 2NP形成5-氯-2-甲基苯并恶唑。
Decolourization, detoxification and biotransformation of 4-chloro-2-nitrophenol (4C2NP) by Bacillus sp. strain MW-1 were studied. This strain decolorized 4C2NP only in the presence of an additional carbon source. On the basis of thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), 4-chloro-2-aminophenol, 4-chloro-2-acetaminophenol and 5-chloro-2-methylbenzoxazole were identified as metabolites. Resting cells depleted 4C2NP with stoichiometric formation of 5-chloro-2-methyl benzoxazole. This is the first report of the formation of 5-chloro-2-methylbenzoxazole from 4C2NP by any bacterial strain.