Carotid repair using autologous adipose-derived endothelial cells.
Carotid repair using autologous adipose-derived endothelial cells.
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DOI：
10.1161/strokeaha.108.539932
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发表时间：
2009-05
期刊：
影响因子：
8.3
通讯作者：
Simari RD
中科院分区：
文献类型：
--
作者：
Froehlich H;Gulati R;Boilson B;Witt T;Harbuzariu A;Kleppe L;Dietz AB;Lerman A;Simari RD
脂肪组织是内皮细胞以及干细胞和前体细胞的丰富来源，可以形成内皮表型。已有研究表明，这些细胞在体外和体内均具有明显的血管生成特性。然而，这些细胞是否有能力直接改善血管损伤后大血管的形态和功能仍不清楚。为了确定脂肪来源的内皮细胞(ADECs)是否能促进受损颈动脉的愈合，采用了一种兔急性动脉损伤模型。利用特定的培养条件制备自体兔ADECs。为了测试ADECS促进颈动脉修复的能力，细胞在急性球囊损伤后被动脉内输送。在分娩前对细胞进行功能选择后，进行了额外的分娩研究。在兔大网膜脂肪采集和消化后，鉴定出增殖的、均一的和明显的内皮细胞群。与生理盐水对照组相比，在急性血管损伤后48小时，直接注射自体ADEC导致显著的再内皮化(82.2±26.9%vs4.2±3.0%p<；0.001)。ADECs是根据其摄取乙酰化低密度脂蛋白的能力而被选择的，它们的输送显著改善了血管的反应性，并减少了血管损伤后的内膜形成。综上所述，这些数据表明，在颈动脉损伤模型中，ADECs代表了一种自体来源的增殖内皮细胞，显示了快速促进再内皮化、改善血管反应性和减少内膜形成的能力。
Adipose tissue is an abundant source of endothelial cells as well as stem and progenitor cells which can develop an endothelial phenotype. It has been demonstrated that these cells have distinct angiogenic properties in vitro and in vivo. However, whether these cells have the capacity to directly improve large vessel form and function following vascular injury remains unknown. To define whether delivery of adipose-derived endothelial cells (ADECs) would improve healing of injured carotid arteries, a rabbit model of acute arterial injury was employed. Autologous rabbit ADECS were generated utilizing defined culture conditions. To test the ability of ADECs to enhance carotid artery repair, cells were delivered intra-arterially following acute balloon injury. Additional delivery studies were performed following functional selection of cells prior to delivery. Following rabbit omental fat harvest and digestion, a proliferative, homogenous, and distinctly endothelial population of ADECs was identified. Direct delivery of autologous ADECs resulted in marked re-endothelialization 48 hours following acute vascular injury as compared to saline controls (82.2 ±26.9% vs 4.2±3.0% p<0.001). Delivery of ADECs that were selected for their ability to take up acetylated LDL significantly improved vasoreactivity and decreased intimal formation following vascular injury. Taken together, these data suggest that ADECs represent an autologous source of proliferative endothelial cells which demonstrate the capacity to rapidly improve re-endothelialization, improve vascular reactivity, and decrease intimal formation in a carotid artery injury model.