Expression of Sirt1 and Sirt2 in injured optic retina of calorie restricted rats.
Expression of Sirt1 and Sirt2 in injured optic retina of calorie restricted rats.
复制标题
Sirt1 和 Sirt2 在热量限制大鼠视神经损伤损伤中的表达。
DOI：
10.3969/j.issn.1000-4432.2011.04.008
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ye Zhi
中科院分区：
文献类型：
--
作者：
Yiqun Geng;Jingying Wang;Jiajian Liang;Ciyan Xu;Ye Zhi
目的
研究卡路里限制(CR)大鼠视神经损伤后视网膜神经节细胞(RGC)的存活情况，并分析sirtuins的潜在作用。
方法
10月龄雄性SD大鼠14只，随机分为热量限制(CR)组和自由采食(AL)组。在CR组(n=7)，每隔一天禁止大鼠进食。AL组(n=7)动物可自由进食。所有受试者于喂养5个月后行右眼PN-ON植入术。术后3周，收集视网膜标本，其中一半用4%多聚甲醛(PFA)固定，行TUJ-1免疫荧光染色。其余样本进行总RNA分析和RT-PCR检测Sirt1和Sirt2的表达。
结果
两组间TUJ-1染色结果比较，RGCs平均密度和细胞总数无显著差异。RT-PCR结果显示，CR组Sirtuin2的mRNA表达明显低于AL组，而Sirt1mRNA的表达在两组间无统计学差异。
结论
卡路里限制对受损视网膜节细胞的存活没有影响。CR组SIRT2的mRNA表达下调可能表明这些动物的再生能力有所提高，但还需要更多的工作来探索这种可能性。
PURPOSE
To investigate retinal ganglion cell (RGC) survival after optic nerve injury in calorie-restricted (CR) rats, and analyze the potential role of Sirtuins.
METHODS
Ten-month old male Sprague-Dawley rats (n=14) were divided into calorie restricted (CR) and ad libitum (AL) groups. In the CR group (n=7), the rats were denied access to food every other day. Animals in the AL group (n=7) had had free access to food. PN-ON grafting was carried out on the right eyes of all subjects after 5 months of feeding. Three weeks postoperatively, retina samples were collected, half of which were fixed in 4% paraformaldehyde (PFA) and subjected to standard immunofluorescence staining for TUJ-1. The remaining samples were subjected to total RNA analysis and RT-PCR for Sirt1 and 2 expression.
RESULTS
Comparing the amount of TUJ-1 staining between the groups, the mean density and the total number of RGCs showed no significant difference. RT-PCR results indicated that mRNA expression of Sirtuin2 in the CR group was significantly lower than that in the AL group, whereas no statistically-significant difference was observed between the two groups regarding the mRNA expression of Sirt1.
CONCLUSION
Calorie restriction had no impact on the survival of injured RGCs. The down-regulated mRNA expression of Sirt2 in the CR group may indicate an improved capacity for regeneration among these animals, but more work is needed to explore this possibility.