A chemiluminescence method to detect malondialdehyde in plasma and urine.
A chemiluminescence method to detect malondialdehyde in plasma and urine.
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DOI：
10.1016/j.ab.2013.08.011
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发表时间：
2013-12
影响因子：
2.9
通讯作者：
Guangbin Zhang;Yuhai Tang;Xiaoyu Shi;Ruixia Gao;Yang Sun;Wei Du;Qiang Fu
中科院分区：
文献类型：
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作者：
Guangbin Zhang;Yuhai Tang;Xiaoyu Shi;Ruixia Gao;Yang Sun;Wei Du;Qiang Fu
采用高效液相色谱-化学发光法建立了一种简便、灵敏的测定血浆和尿液中丙二醛（MDA）的方法。通过反相C18柱以0.8 ml min-1的流速分离MDA。然后通过化学发光检测器检测。考察了移动的相、流速、化学发光试剂、光电倍增管参数和温度等因素对化学发光反应的影响。该方法在0.50至50 μmol L−1范围内呈线性，检测限和定量限分别为0.08和0.30 μmol L−1。回收率在92.2%至98.5%之间，日内和日间重现性得到的相对标准偏差小于4%和小于6%，分别。血浆和尿液中的MDA在4 °C下稳定12 h。血浆MDA水平分别为2.74、3.19和3.20 μmol L− 1，尿液MDA水平分别为1.25、2.04和1.58 μmol L− 1。该方法简单、快速、灵敏、成本低。该方法适用于组织和饲料中MDA的检测。
A highly sensitive and convenient method to detect malondialdehyde (MDA) in specimens of plasma and urine was developed using high-performance liquid chromatography combining chemiluminescence detection. MDA was separated through a reverse-phase C18 column at a flow rate of 0.8 ml min−1. It was then detected by a chemiluminescence detector. Variables that affected chemiluminescence reaction, including mobile phase, flow rate, chemiluminescence reagent, parameters of the photomultiplier tube, and temperature, were studied. This assay was linear from 0.50 to 50 μmol L−1, with limits of detection and quantification of 0.08 and 0.30 μmol L−1, respectively. The recoveries were in the range of 92.2 to 98.5%, and the intra- and interday reproducibilities were obtained with relative standard deviations of less than 4% and less than 6%, respectively. MDA in plasma and urine was stable for 12 h at 4 °C. MDA levels were 2.74, 3.19, and 3.20 μmol L−1for plasma and 1.25, 2.04, and 1.58 μmol L−1for urine, respectively. The proposed method is simple, rapid, and sensitive with low cost. Taken together, this method can be suitable for detecting MDA in tissue and feed specimens.