Labeling and Single-Particle-Tracking-Based Entry Mechanism Study of Vaccinia Virus from the Tiantan Strain
Labeling and Single-Particle-Tracking-Based Entry Mechanism Study of Vaccinia Virus from the Tiantan Strain
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天坛株痘苗病毒标记与单粒子追踪侵入机制研究
DOI：
10.1021/acs.analchem.7b05183
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发表时间：
2018-03-06
影响因子：
7.4
通讯作者：
Xie, Hai-Yan
中科院分区：
文献类型：
--
作者：
Huang, Li-Li;Wu, Li-Li;Xie, Hai-Yan
病毒进入是病毒感染的第一步和关键步骤，由于缺乏有效的技术，许多病毒的进入机制尚不清楚。本报告利用所研制的单病毒粒子荧光跟踪技术和病毒同步双标记技术，对天坛株牛痘病毒（VACV-TT）的进入途径进行了实时研究。结合病毒学和细胞生物学技术，我们发现VACV-TT在病毒粒子相互作用诱导下沿丝状伪足向Vero细胞体移动，然后通过巨噬细胞作用被内化，这是一种依赖于肌动蛋白、atp而不依赖于网格蛋白、小窝蛋白的内吞作用。这些结果对基于vacv - tt的疫苗载体和溶瘤病毒的研究具有重要意义。
Entry is the first and critical step of viral infection, while the entry mechanisms of many viruses are still unclear due to the lack of efficient technology. In this report, by taking advantage of the single-virion fluorescence tracking technique and simultaneous dual-labeling methods for viruses we developed, the entry pathway of vaccinia virus from tiantan strain (VACV-TT) was studied in real-time. By combining with the technologies of virology and cell biology, we found that VACV-TT moved toward the Vero cell body along the filopodia induced by the virions interaction, and then, they were internalized through macropinocytosis, which was an actin-, ATP-dependent but clathrin-, caveolin-independent endocytosis. These results are of significant importance for VACV-TT-based vaccine vectors and oncolytic virus study.