Interleukin 2 is both necessary and sufficient for the growth and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.
Interleukin 2 is both necessary and sufficient for the growth and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.
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白细胞介素 2 对于凝集素刺激的溶细胞 T 淋巴细胞前体的生长和分化来说是必要且充分的。
DOI：
10.4049/jimmunol.134.3.1644
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发表时间：
1985
影响因子：
4.4
通讯作者：
H. Macdonald
中科院分区：
文献类型：
--
作者：
F. Erard;P. Corthésy;M. Nabholz;J. Lowenthal;P. Zaech;G. Plaetinck;H. Macdonald
研究了凝集素刺激的溶细胞性T淋巴细胞前体（CTL-P）生长和分化的最低要求。通过用单克隆抗Lyt-2抗体染色，然后在荧光激活的细胞分选仪上进行阳性选择，从小鼠脾脏纯化CTL-P。用免疫亲和层析或重组DNA技术获得的凝集素（白细胞凝集素或伴刀豆球蛋白A）和纯白细胞介素2（IL 2）的最佳浓度建立含有2000个纯化Lyt-2+细胞的微培养物。凝集素刺激的Lyt-2+细胞的增殖和CTL活性的产生具有IL-2剂量依赖性。此外，没有增加的反应时，观察到含有其他推定的CTL分化因子的粗上清液中加入，即使当IL 2浓度是次优的。当以有限稀释度培养被1% Lyt-2-细胞和少于0.1%巨噬细胞污染的高纯度Lyt-2+细胞时，在白细胞凝集素和纯IL 2的存在下，只有20个响应细胞增殖并分化为CTL;事实上，在单个微量培养物中，CTL活性和增殖密切相关。此外，在这些实验条件下，10%至30%的个体CTL-P是生长诱导型的。总之，这些数据表明，凝集素刺激的CTL-P的增殖和分化可以在纯IL 2中发生，而不需要任何可证明的其他分化因子或辅助细胞。
Minimal requirements for the growth and differentiation of lectin-stimulated cytolytic T lymphocyte precursors (CTL-P) have been investigated. CTL-P from murine spleen were purified by staining with monoclonal anti-Lyt-2 antibodies, followed by positive selection on a fluorescence-activated cell sorter. Microcultures containing 2000 purified Lyt-2+ cells were set up with optimal concentrations of lectin (leukoagglutinin or concanavalin A) and pure interleukin 2 (IL 2) obtained either by immunoaffinity chromatography or recombinant DNA technology. Proliferation and generation of CTL activity in lectin-stimulated Lyt-2+ cells were IL 2 dose-dependent. Furthermore, no increased response was observed when crude supernatants containing other putative CTL differentiation factors were added, even when IL 2 concentrations were suboptimal. When highly purified Lyt-2+ cells contaminated by 1% Lyt-2- cells and less than 0.1% macrophages were cultured at limiting dilution, as few as 20 responding cells proliferated and differentiated into CTL in the presence of leukoagglutinin and pure IL 2; in fact, CTL activity and proliferation were strongly correlated in individual microcultures. In addition, 10 to 30% of individual CTL-P were growth-inducible under these experimental conditions. Together, these data show that the proliferation and differentiation of lectin-stimulated CTL-P can occur in pure IL 2 without any demonstrable requirement for other differentiation factors or accessory cells.