An enzymatic method for the measurement of glycated albumin in biological samples
An enzymatic method for the measurement of glycated albumin in biological samples
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DOI：
10.1016/s0009-8981(02)00207-3
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发表时间：
2002-10-01
影响因子：
5
通讯作者：
Imamura, S
中科院分区：
文献类型：
--
作者：
Kouzuma, T;Usami, T;Imamura, S
背景：为了更简便、快速地测定糖化白蛋白，我们开发了一种用于血液样本中糖化白蛋白的新型酶法。
方法：该方法使用白蛋白特异性蛋白酶、酮胺氧化酶和血清白蛋白检测试剂。在检测过程中，糖化白蛋白通过蛋白酶消化水解为糖化氨基酸，酮胺氧化酶将糖化氨基酸氧化产生过氧化氢，然后对其进行定量检测。糖化白蛋白以其在总白蛋白中所占百分比来计算。
结果：糖化白蛋白浓度的校准曲线在0.0 - 50.0 g/L之间呈线性（r(p)=0.999），白蛋白浓度的校准曲线在0.0 - 60.0 g/L之间呈线性（r(p)=0.999）。添加到血清中的外源性糖化白蛋白的分析回收率为100 - 102.5%。批内和批间变异系数分别为0.45 - 0.67%和1.09 - 1.26%。该方法不受胆红素、乳糜、葡萄糖、球蛋白和不稳定中间体的干扰。观察到血红蛋白和抗坏血酸有轻微干扰。本方法检测的糖化白蛋白与高效液相色谱（HPLC）法检测的糖化白蛋白显著相关（血清：r(s)=0.989，血浆：r(p)=0.992）。
结论：这种新型酶法简单、快速，可进行多次测定，并能对糖化白蛋白进行定量分析。（C）2002 Elsevier Science B.V. 保留所有权利。
Background: In order to determine glycated albumin more easily and rapidly, we developed a new enzymatic method for glycated albumin in blood samples. Methods: The method involves use of albumin-specific proteinase, ketoamine oxidase and serum albumin assay reagent. In the assay, glycated albumin is hydrolyzed to glycated amino acids by proteinase digestion, and ketoamine oxidase oxidizes the glycated amino acids to produce hydrogen peroxide, which is quantitatively measured. Glycated albumin is calculated as the percentage of glycated albumin in total albumin. Results: The calibration curve for glycated albumin concentration was linear (r(p)=0.999) between 0.0 and 50.0 g/l and that for albumin concentration was linear (r(p)=0.999) between 0.0 and 60.0 g/l. The analytical recoveries of exogenous glycated albumin added to serum were 100-102.5%. The within-run and between-run CVs were 0.45-0.67% and 1.09-1.26%, respectively. This method was free from interference by bilirubin, chyle, glucose, globulins and labile intermediate. Weak interference by hemoglobin and ascorbic acid was observed. Glycated albumin detected by the present method was significantly correlated with glycated albumin detected by high-performance liquid-chromatographic (HPLC) method (serum: r(s)=0.989, plasma: r(p)=0.992). Conclusions: This new enzymatic method is simple, rapid, allows multiple determinations and enables quantitative analysis of glycated albumin. (C) 2002 Elsevier Science B.V. All rights reserved.