Oils Derived from GM Crops as Sustainable Solutions to the Supply of Long-Chain Omega-3 for On-Growing Atlantic Bluefin Tuna (Thunnus thynnus L.)
Oils Derived from GM Crops as Sustainable Solutions to the Supply of Long-Chain Omega-3 for On-Growing Atlantic Bluefin Tuna (Thunnus thynnus L.)
复制标题
DOI：
10.3390/fishes7060366
复制
发表时间：
2022-11
期刊：
影响因子：
2.3
通讯作者：
M. B. Betancor;M. Sprague;D. González-Silvera;A. Ortega;F. de la Gándara;Xupeng Gong;J. Napier;D. Tocher;G. Mourente
中科院分区：
文献类型：
--
作者：
M. B. Betancor;M. Sprague;D. González-Silvera;A. Ortega;F. de la Gándara;Xupeng Gong;J. Napier;D. Tocher;G. Mourente
最近，亚麻荠经过基因改造，生产出富含 omega-3 (n-3) 长链多不饱和脂肪酸 (LC-PUFA)、EPA（二十碳五烯酸）和 EPA + DHA（二十二碳六烯酸）的油。本研究的目的是测试使用这些新型 EPA 和 EPA + DHA 来源作为大西洋蓝鳍金枪鱼幼鱼 (ABT) 饲料中海洋油的替代品的可行性。结果表明，这些油是 n-3 LC-PUFA 的实用来源，有可能取代 ABT 幼鱼饲料中的鱼油 (FO)。饲喂测试日粮（ECO、单独 EPA 和 DCO、EPA + DHA）的鱼表现出良好的生长性能、存活率和饲料利用率，接近饲喂含有海洋鱼油的参考日粮 (MGK) 的 ABT，排名顺序为 MGK > DCO > ECO。测试饮食显示出积极的效果，上调了主要核受体基因的表达以及脂质代谢（包括消化、LC-PUFA 合成和抗氧化途径）的基因表达。结果表明，同时含有 DHA 和 EPA 的 DCO 饲料比 DHA 含量低得多的 ECO 饲料表现更好。然而，用这两种油配制的饲料可能仍需要补充 DHA 以满足 ABT 对这种必需营养素的高要求。
Recently Camelina sativa, has been genetically modified to produce oils rich in omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), EPA (eicosapentaenoic acid) and EPA + DHA (docosahexaenoic acid). The aim of this study was to test the feasibility of using these novel sources of de novo EPA and EPA + DHA as substitutes for marine oil in feeds for juvenile Atlantic Bluefin tuna (ABT). The results showed the oils were practical sources of n-3 LC-PUFA which could potentially replace fish oil (FO) in feeds for ABT juveniles. Fish fed the test diets (ECO, EPA alone and DCO, EPA + DHA) displayed good growth performance, survival and feed utilisation approaching that of ABT fed the reference diet (MGK) containing marine fish oil with the rank order being MGK > DCO > ECO. The test diets showed positive effects, upregulating the expression of genes of major nuclear receptors and those of lipid metabolism including digestion, LC-PUFA synthesis and antioxidant pathways. The results indicated that the DCO feed containing both DHA and EPA performed better than the ECO feed with much lower DHA. However, feeds formulated with both these oils may still require supplementary DHA to satisfy the high requirement of ABT for this essential nutrient.