Antidepressant effect of recombinant NT4-NAP/AAV on social isolated mice through intranasal route.
Antidepressant effect of recombinant NT4-NAP/AAV on social isolated mice through intranasal route.
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重组NT4-NAP/AAV通过鼻内途径对社交隔离小鼠的抗抑郁作用
DOI：
10.18632/oncotarget.14356
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发表时间：
2017-02-07
期刊：
影响因子：
--
通讯作者：
Dang YH
中科院分区：
文献类型：
--
作者：
Liu F;Liu YP;Lei G;Liu P;Chu Z;Gao CG;Dang YH
本研究的目的是观察社会隔离引起的抑郁症行为。检测通过鼻内递送对社会隔离小鼠的小睡的重组腺相关病毒（AAV）的抗抑郁作用。构建NT4-NAP/AAV后，在体外证实了NAP的表达。将3周龄的C57/BL小鼠单独繁殖为社会隔离。六个星期后，小鼠的第一个子集接受了行为测试和蛋白质印迹。第二个是用于酶联免疫吸附测定法。行为测试前10天，通过鼻施用NT4-NAP/AAV将Quaque Die交付。在社会隔离小鼠中观察到了几种类似抑郁症的行为，包括相对的蔗糖偏好减少，强迫游泳测试中的固定时间较长，血浆皮质酮较低和海马中脑衍生的神经营养因子降低。因此，社会隔离程序似乎是一种具有良好脸和构造有效性的抑郁症动物模型。更重要的是，在NT4-NAP/AAV鼻内给药后观察到了社会隔离小鼠的抗抑郁作用，这表明这可能是抑郁症的有希望的治疗策略。
The purpose of the present study was to observe the depression-like behavior induced by social isolation; detect the antidepressant effect of a recombinant adeno-associated virus (AAV) expressing NAP on social isolation mice by intranasal delivery. After construction of NT4-NAP/AAV, expression of NAP was confirmed in vitro. 3-week-old C57/BL mice were bred individually in cages as social isolation-rearing. Six weeks later, the first subset of mice underwent behavioral tests and western blot; the second was for enzyme-linked immunosorbent assay. NT4-NAP/AAV was delivered quaque die by nasal administration for consecutive 10 days before behavioral test. Several depression-like behaviors were observed in social isolation mice, including decreased relative sucrose preference, longer immobility time in forced swimming test, lower plasma corticosterone and decreased brain-derived neurotrophic factor in hippocampus. Thus, social isolation procedure appears to be an animal model of depression with good face and construct validity. What's more, the antidepressant effect in social isolation-rearing mice was observed after intranasal administration of NT4-NAP/AAV, suggesting that this might be a promising therapeutic strategy for depressive disorder.