Comparative assessment of distribution of blackcurrant anthocyanins in rabbit and rat ocular tissues
Comparative assessment of distribution of blackcurrant anthocyanins in rabbit and rat ocular tissues
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DOI：
10.1016/j.exer.2005.12.019
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发表时间：
2006-08-01
影响因子：
3.4
通讯作者：
Ohguro, Hiroshi
中科院分区：
文献类型：
--
作者：
Matsumoto, Hitoshi;Nakamura, Yuko;Ohguro, Hiroshi
花青素是一类广泛存在于水果和蔬菜中的酚类化合物。尽管食用这些化合物可以改善视觉功能，但尚未详细研究ACS在眼组织中的分布。因此，本研究的目的是评价黑加仑花色苷(BCA)在大鼠和兔体内的眼部分布。和腹膜腔(I.P.)行政管理。分别采用高效液相色谱-电喷雾电离-质谱法(HPLC-ESI-MS)和高效液相色谱-紫外-可见检测法(HPLCUV-Vis)对其进行鉴定和定量。口服和腹腔注射后，血浆和全眼组织中均可检出BCA。大鼠给药。当在520 nm处监测洗脱液的吸光度时，在给药后的血浆或眼组织中均未检测到其他峰。这一发现表明，在给予BCA后，大鼠存在完整形式的急性冠脉综合征。在给大鼠ip。给药后，全眼和部分眼组织中的总ACS浓度高于血浆浓度。这些结果表明，在眼组织中检测到的急性冠脉综合征不是由于残血造成的。跟随静脉注射。在兔体内，在血浆和房水、角膜、巩膜、脉络膜、睫状体、虹膜和视网膜等几种眼组织中发现了四种ACS。在玻璃体和晶状体中也检测到少量的急性冠脉综合征。综上所述，本研究证明BCA在眼组织中以完整的形式被吸收和分布。我们的数据清楚地表明，在大鼠和兔体内，完整形式的支链动脉都可以通过血-房水屏障和血-视网膜屏障。(C)2006爱思唯尔有限公司。保留所有权利。
Anthocyanins (ACs) are phenolic compounds that are distributed widely in fruits and vegetables. Although consumption of these compounds has been shown to improve visual function, the distribution of ACs in ocular tissue has not been examined in detail. The aim of this study was therefore to evaluate the ocular distribution of blackcurrant anthocyanins (BCAs) in rats and rabbits after oral, intravenous (i.v.) and intraperitoneal (i.p.) administration. Identification and quantification of ACs were carried out using high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) and high-performance liquid chromatography (HPLC) with UV-visible detection, respectively. BCAs were identified in the plasma and whole eye after oral and i.p. administration in rats. No other peaks were detected in either plasma or ocular tissues after administration when the absorbance of the eluate was monitored at 520 nm. This finding indicates that intact forms of ACs were present in rats after administration of BCA. In rats given i.p. administration, the concentration of total ACs in the whole eye and some ocular tissues was higher than that measured in plasma. These results suggested that ACs detected in the ocular tissues were not due to residual blood. Following i.v. administration in rabbits, four ACs were identified in the plasma and several ocular tissues including the aqueous humor, cornea, sclera, choroid, ciliary body, iris and retina. A small amount of ACs was also detected in the vitreous and lens. In conclusion, this study demonstrated that BCAs were absorbed and distributed in ocular tissues as intact forms. Our data show clearly that intact forms of BCAs pass thorough the blood-aqueous barrier and blood-retinal barrier in both rats and rabbits. (c) 2006 Elsevier Ltd. All rights reserved.