Construction of a cDNA microarray derived from the ascidian Ciona intestinalis
Construction of a cDNA microarray derived from the ascidian Ciona intestinalis
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DOI：
10.2108/zsj.20.1223
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发表时间：
2003-10-01
影响因子：
0.9
通讯作者：
Satoh, N
中科院分区：
文献类型：
--
作者：
Azumi, K;Takahashi, H;Satoh, N
构建了一种以肠囊海鞘为基础的基因芯片。已阅读和推断的芝麻属基因组草案包含类似于16,000个蛋白质编码基因，13,464个基因的转录本的cDNA已被鉴定和编辑为“芝麻属基因收集发布I”。在本研究中，我们构建了这13,464个Ciona基因的cDNA微阵列。用Cy3和Cy5标记的探针进行的初步实验表明，受精卵和幼虫之间存在广泛的差异基因表达。此外，目前的微阵列分析结果与以前的EST分析结果之间有很好的相关性。这是第一个收集了一大批肠黄瓜基因克隆的微阵列，应该有助于分析基础脊索动物胚胎发生过程中的整体基因表达和基因网络。
A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain similar to16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.