Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia
Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia
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流式细胞术检测CD71表达对急性白血病的诊断意义
DOI：
10.3109/10428194.2013.819100
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发表时间：
2014-04-01
影响因子：
2.6
通讯作者：
Zhu, Ping
中科院分区：
文献类型：
--
作者：
Liu, Qian;Wang, Mangju;Zhu, Ping
摘要：在本研究中，我们探讨了 CD71（转铁蛋白受体 1，TfR-1）作为流式细胞术标记物在急性白血病 (AL) 诊断中的意义。共有 105 名 AL 患者入组。低分化急性髓系白血病（AML）（包括微分化AML、未成熟AML、成熟AML、急性粒单核细胞白血病）往往在白血病细胞上表达高水平的CD71，而部分分化AML（包括急性早幼粒细胞白血病和急性单核细胞白血病）往往在白血病细胞上表达低水平CD71 细胞（与低分化 AML 相比，p < 0.05）。 B细胞急性淋巴细胞白血病（B-ALL）在白血病细胞上表达低水平的CD71，显着低于AML、混合表型急性白血病（MPAL）和正常骨髓母细胞（p < 0.05）。 7例急性红系白血病（AEL）中，白血病细胞很少表达CD71，平均CD71表达水平显着低于急性巨核细胞白血病（p < 0.05），也低于低分化AML和正常母细胞，但无统计学意义。 CD71 可能不是 AEL 白血病细胞的特异性标志物。在从髓系发育不良到明显白血病细胞的过程中，CD71和CD34均逐渐增加。因此，具有不同水平的 CD71 和 CD34 的白血病细胞亚群的存在可能有助于理解白血病中克隆发育所涉及的动态过程。
Abstract In this study we investigated the significance of CD71 (transferrin receptor 1, TfR-1) as a flow cytometric marker in the diagnosis of acute leukemia (AL). A total of 105 patients with AL were enrolled. Poorly differentiated acute myeloid leukemias (AMLs) (including minimally differentiated AML, AML without maturation, AML with maturation, acute myelomonocytic leukemia) tended to express high levels of CD71 on leukemic cells, while partially differentiated AML (including acute promyelocytic leukemia and acute monocytic leukemia) often expressed low levels of CD71 on leukemic cells (p < 0.05, compared to poorly differentiated AML). B-cell acute lymphoblastic leukemia (B-ALL) expressed low levels of CD71 on leukemic cells, significantly lower than AML, mixed phenotype acute leukemia (MPAL) and normal bone marrow blasts (p < 0.05). In the seven cases of acute erythroid leukemia (AEL), leukemic cells rarely expressed CD71, with the mean CD71 expression level significantly lower than that of acute megakaryocytic leukemia (p < 0.05), and also lower than that of poorly differentiated AML and normal blasts but without statistical significance. CD71 may not be a specific marker for AEL leukemic cells. During the process from myeloid dysplasia to apparent leukemic cells, both CD71 and CD34 gradually increased. Consequently, the presence of leukemic cell subsets with variable levels of CD71 and CD34 may be useful for understanding the dynamic processes involved in the clonal development seen in leukemias.