The MYO1F, unconventional myosin type 1F, gene is fused to MLL in infant acute monocytic leukemia with a complex translocation involving chromosomes 7, 11, 19 and 22
The MYO1F, unconventional myosin type 1F, gene is fused to MLL in infant acute monocytic leukemia with a complex translocation involving chromosomes 7, 11, 19 and 22
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DOI：
10.1038/sj.onc.1208711
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发表时间：
2005-08
期刊：
影响因子：
8
通讯作者：
T. Taki;M. Akiyama;S. Saito;R. Ono;M. Taniwaki;Y. Kato;Y. Yuza;Y. Eto;Y. Hayashi
中科院分区：
文献类型：
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作者：
T. Taki;M. Akiyama;S. Saito;R. Ono;M. Taniwaki;Y. Kato;Y. Yuza;Y. Eto;Y. Hayashi
我们分析了婴儿急性单核细胞白血病中涉及染色体7、11、19和22的复杂易位，并确定11 q23上的MLL基因与19 p13上的非常规肌球蛋白1F型MYO 1F基因融合。2-13.3. MYO 1F由至少28个外显子组成，预测编码1098个氨基酸，N端头部结构域包含ATP结合序列和肌动蛋白结合序列，颈部结构域包含单个IQ基序，尾部结构域包含TH 1、TH 2和SH 3结构域。从多个组织制备的RNA的北方印迹分析表明，在大多数检查的组织中，大约4-kb的转录物的表达似乎是恒定的。然而，MYO 1F仅在22个白血病细胞系中的3个中表达。MLL-MYO 1F融合蛋白包含几乎整个MYO 1F，然而，C-末端MYO 1F既没有反式激活结构域，也没有在各种MLL融合伴侣中发现的二聚化结构域。对这种新型MLL融合蛋白的进一步分析将为白血病的发生提供新的见解。MYO 1F是MLL的第四个伴侣基因，位于19 p13。在细胞遗传学水平上，可能难以区分由t（11; 19）（q23; p13）产生的MLL-ENL、MLL-ELL、MLL-EEN和MLL-MYO 1F融合，并且很可能缺乏已知融合基因的t（11; 19）病例可能导致这种基因融合。
We analysed a complex translocation involving chromosomes 7, 11, 19 and 22 in infant acute monocytic leukemia, and identified that the MLL gene on 11q23 was fused to the unconventional myosin type 1F, MYO1F, gene on 19p13. 2–13.3. MYO1F consists of at least 28 exons and was predicted to encode a 1098-amino-acid with an N-terminal head domain containing both ATP-binding and actin-binding sequences, a neck domain with a single IQ motif, and a tail with TH1, TH2 and SH3 domains. Northern blot analysis of RNAs prepared from multiple tissues showed that the expression of approximately 4-kb transcripts appeared constant in most tissues examined. However, MYO1F was expressed in only three of 22 leukemic cell lines. The MLL-MYO1F fusion protein contains almost the entire MYO1F, however, C-terminal MYO1F has neither the transactivation domain nor the dimerization domain found in various MLL fusion partners. Further analysis of this novel type of MLL fusion protein would provide new insights into leukemogenesis. MYO1F is the fourth partner gene of MLL on 19p13. At the cytogenetic level, it may be difficult to distinguish MLL-ENL, MLL-ELL, MLL-EEN and MLL-MYO1F fusions created by t (11; 19)(q23; p13), and it is likely that cases of t (11; 19) lacking a known fusion gene may result in this gene fusion.