Compressed sensing laser scanning microscopy
Compressed sensing laser scanning microscopy
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DOI：
10.1364/oe.24.030038
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发表时间：
2016-12-26
期刊：
影响因子：
3.8
通讯作者：
Smith, N. I.
中科院分区：
文献类型：
--
作者：
Pavillon, N.;Smith, N. I.
我们提出了一种基于压缩感知的激光扫描显微镜的测量和重建方法，该方法可以显着提高帧速率并减少光漂白。通过将物理成像过程的模型包括到压缩感知重建过程中来确保图像重建精度。我们证明了它的适用性，未经修改的商业共聚焦荧光显微镜系统和拉曼成像，显示出潜在的数据减少10-15倍，这直接导致提高采集速度，或减少光漂白，而不会显着损失的空间分辨率。此外，重建模型对噪声也是鲁棒的，并且对于弱光应用是有效的。该方法具有良好的应用前景的所有成像模式的基础上，激光扫描采集，包括荧光，拉曼，和非线性显微镜。(C)2016美国光学学会
We present a measurement and reconstruction method for laser-scanning microscopy based on compressed sensing, which enables significantly higher frame rates and reduced photobleaching. The image reconstruction accuracy is ensured by including a model of the physical imaging process into the compressed sensing reconstruction procedure. We demonstrate its applicability to unmodified commercial confocal fluorescence microscopy systems and for Raman imaging, showing a potential data reduction of 10-15 times, which directly leads to improvements in acquisition speed, or reduction of photobleaching, without significant loss of spatial resolution. Furthermore, the reconstruction model is also robust to noise, and effective for low-light applications. This method has promising applications for all imaging modalities based on laser-scanning acquisition, including fluorescence, Raman, and nonlinear microscopy. (C) 2016 Optical Society of America