On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes.
On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes.
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DOI：
10.1038/srep36498
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发表时间：
2016-11-04
影响因子：
4.6
通讯作者：
Spira ME
中科院分区：
文献类型：
--
作者：
Rabieh N;Ojovan SM;Shmoel N;Erez H;Maydan E;Spira ME
与微电极阵列（MEA）技术在培养神经元和心肌的电生理研究中的广泛应用相反，骨骼肌研究的广阔领域尚未采用该技术。在这里，我们展示了一种授权MEA技术，用于培养骨骼肌肌管的高质量，多位点，长期电生理记录。在以金蘑菇形微电极（gMμE）为基底的微电极上培养的大鼠骨骼肌肌管能紧密地吞噬gMμ E，在肌管和gMμ E之间形成高的密封阻力。因此，由收缩肌管产生的自发动作电位被记录为幅度高达10 mV的胞外场电位，持续14天以上。通过gMμEs施加10 ms，0.5-0.9 V电压脉冲电穿孔肌管膜，并将细胞外记录模式瞬时转换为细胞内记录模式10-30 min。在一小部分的文化稳定衰减的细胞内记录自发产生。在这些情况下或电穿孔后，还记录了阈下自发电位。gMμE-MEA作为一种简单易用的高质量电生理工具的引入，以及在使用培养的人类肌管方面取得的进展，为基础和临床骨骼肌研究、临床前药物筛选和个性化医疗开辟了新的途径。
In contrast to the extensive use of microelectrode array (MEA) technology in electrophysiological studies of cultured neurons and cardiac muscles, the vast field of skeletal muscle research has yet to adopt the technology. Here we demonstrate an empowering MEA technology for high quality, multisite, long-term electrophysiological recordings from cultured skeletal myotubes. Individual rat skeletal myotubes cultured on micrometer sized gold mushroom-shaped microelectrode (gMμE) based MEA tightly engulf the gMμEs, forming a high seal resistance between the myotubes and the gMμEs. As a consequence, spontaneous action potentials generated by the contracting myotubes are recorded as extracellular field potentials with amplitudes of up to 10 mV for over 14 days. Application of a 10 ms, 0.5–0.9 V voltage pulse through the gMμEs electroporated the myotube membrane, and transiently converted the extracellular to intracellular recording mode for 10–30 min. In a fraction of the cultures stable attenuated intracellular recordings were spontaneously produced. In these cases or after electroporation, subthreshold spontaneous potentials were also recorded. The introduction of the gMμE-MEA as a simple-to-use, high-quality electrophysiological tool together with the progress made in the use of cultured human myotubes opens up new venues for basic and clinical skeletal muscle research, preclinical drug screening, and personalized medicine.
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影响因子：
3.8
作者：
Horn, R;Marty, A
通讯作者：
Marty, A
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
64.8
作者：
Itzhaki, Ilanit;Maizels, Leonid;Gepstein, Lior
通讯作者：
Gepstein, Lior
DOI：
10.3389/fneng.2011.00014
发表时间：
2011
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
Fendyur A;Mazurski N;Shappir J;Spira ME
通讯作者：
Spira ME
影响因子：
5.5
作者：
GROSSMAN, Y;PARNAS, I;SPIRA, ME
通讯作者：
SPIRA, ME