[Single nucleotide polymorphism analysis in chicken leptin receptor exon 9].
[Single nucleotide polymorphism analysis in chicken leptin receptor exon 9].
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发表时间：
2002-05
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通讯作者：
Z. Gu;Jianguo Zhao;Hui Li;H. Meng;Qigui Wang;Qing-hua Wang;Da-Hai Zhu
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作者：
Z. Gu;Jianguo Zhao;Hui Li;H. Meng;Qigui Wang;Qing-hua Wang;Da-Hai Zhu
瘦素受体是I型细胞因子超家族成员，在瘦素功能信号转导中发挥重要作用。本研究对肥满系(FL)、瘦肉系(LL)、北京油鸡、白耳鸡、石旗杂、矮化黄鸡、迷你黄鸡、惠阳沪徐鸡、隐性白鸡和海兰蛋鸡等品种的OBR基因进行了单核苷酸多态性(SNP)分析。从鸡基因组序列数据库中设计OBR基因外显子9的引物，用聚合酶链式反应-单链构象多态性方法进行SNPs检测。在所有品种的个体中都发现了一个SNP(C/A值为1167)。然而，氨基酸没有改变，因为它是一个沉默突变。群体遗传学分析结果表明，北京油鸡AA基因频率显著高于其他品系。FL组A等位基因频率显著高于LL组。
Leptin receptor is a type I cytokine super family member and plays an important role in leptin functioning signal transduction. This study was designed to investigate the single nucleotide polymorphism (SNP) of OBR gene in various breeds, including Fatness Line (FL), Leanness Line (LL), Beijing Youji, Baierji, Shiqiza, Dwarf Yellow Chickens, Mini Yellow Chickens, Huiyang Huxuji, Recessive White Chickens and Hyline Layer. The primers for exon 9 in OBR gene were designed from the database of chicken genomic sequence and the SNPs were detected by PCR-SSCP method. One SNP (C/A at 1167 in cds) was found among individuals within all breeds. However, the amino acid was not changed because it was a silence mutation. The result of population genetics analyses showed that the frequency of AA genotype in Beijing Youji was significantly higher than that in other lines. Also, the frequency of A allele in FL was significantly higher than that in LL.