In-situ crosslinked wet spun collagen triple helices with nanoscale-regulated ciprofloxacin release capability
In-situ crosslinked wet spun collagen triple helices with nanoscale-regulated ciprofloxacin release capability
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DOI：
10.1016/j.matlet.2019.126550
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发表时间：
2019-11-15
影响因子：
3
通讯作者：
Russell, Stephen J.
中科院分区：
文献类型：
--
作者：
Arafat, M. Tarik;Tronci, Giuseppe;Russell, Stephen J.
设计具有受控药物释放能力的抗菌释放涂层或包装材料是一种有前途的策略，可以最大限度地减少体内感染和医疗器械故障的风险。胶原蛋白纤维已经被用作医疗器械的构建块，尽管它们仍然不能显示出控制释放能力，具有竞争力的湿态机械性能，并保持三螺旋结构。我们通过寻求一种集药物包裹、原位共价交联和纤维纺丝为一体的多尺度设计方法来研究这一挑战。通过选择环丙沙星(Cip)作为一种典型的抗菌药物，湿法纺丝被选为制备Cip包埋的胶原纤维的三螺旋友好路线；而形成纤维的三螺旋与1，3-苯二乙酸(Ph)的原位交联会产生Ph-Cip pi-pi堆积的芳香族相互作用，从而实现药物的可控释放。与最先进的碳二亚胺交联对照相比，在Ph交联纤维中测量到了更高的拉伸模数和强度。与不含CIP的异构体和碳二亚胺交联的对照相比，CIP包裹的Ph交联纤维分别显示出断裂伸长率的降低和体外药物保留率的显著提高。这种多规模的制造策略为湿纺胶原三螺旋提供了新的见解，具有纳米级可调节的拉伸性能和药物释放能力。(C)2019爱思唯尔B.V.保留所有权利。
The design of antibacterial-releasing coatings or wrapping materials with controlled drug release capability is a promising strategy to minimise risks of infection and medical device failure in vivo. Collagen fibres have been employed as medical device building block, although they still fail to display controlled release capability, competitive wet-state mechanical properties, and retained triple helix organisation. We investigated this challenge by pursuing a multiscale design approach integrating drug encapsulation, in-situ covalent crosslinking and fibre spinning. By selecting ciprofloxacin (Cip) as a typical antibacterial drug, wet spinning was selected as a triple helix-friendly route towards Cip-encapsulated collagen fibres; whilst in-situ crosslinking of fibre-forming triple helices with 1,3-phenylenediacetic acid (Ph) was hypothesised to yield Ph-Cip pi-pi stacking aromatic interactions and enable controlled drug release. Higher tensile modulus and strength were measured in Ph-crosslinked fibres compared to state-ofthe-art carbodiimide-crosslinked controls. Cip-encapsulated Ph-crosslinked fibres revealed decreased elongation at break and significantly-enhanced drug retention in vitro with respect to Cip-free variants and carbodiimide-crosslinked controls, respectively. This multiscale manufacturing strategy provides new insight aiming at wet spun collagen triple helices with nanoscale-regulated tensile properties and drug release capability. (C) 2019 Elsevier B.V. All rights reserved.