Loureirin B inhibits the proliferation of hepatic stellate cells and the Wnt/β-catenin signaling pathway by regulating miR-148-3p.
Loureirin B inhibits the proliferation of hepatic stellate cells and the Wnt/β-catenin signaling pathway by regulating miR-148-3p.
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Loureirin B 通过调节 miR-148-3p 抑制肝星状细胞和 Wnt/β-catenin 信号通路的增殖
DOI：
10.1186/s11658-018-0098-9
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发表时间：
2018
影响因子：
8.3
通讯作者：
Song ZJ
中科院分区：
文献类型：
--
作者：
Hu JP;Zhang R;Tang M;Li YL;Xun LT;Shi ZZ;An Y;Li T;Song ZJ
背景我们研究龙血素B抗肝纤维化的活性及其分子机制。方法用不同浓度的龙血素B处理Sprague-Dawley大鼠肝星状细胞（HSC）。采用MTT法检测HSC增殖，流式细胞术检测细胞凋亡，Western blot检测Bax、Bcl-2、Wnt 1和β-catenin的表达。结果MTT法显示龙血素B对HSCs的增殖有明显的抑制作用，且呈时间和剂量依赖性。龙血素B可促进HSC凋亡，增加Bax表达，降低Bcl-2水平。Western blot分析显示龙血素B治疗组Wnt 1和β-catenin的表达明显低于对照组。龙血素B可降低Wnt 1 mRNA水平，增加miR-148- 3 p表达。结论龙血素B通过下调miR-148 - 3 p的表达，抑制HSC增殖和凋亡，抑制Bax、Bcl-2、Wnt 1和β-catenin蛋白的表达，抑制Wnt/β-catenin信号通路。
BackgroundWe investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms.MethodsHepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p.ResultsThe MTT assay showed that loureirin B treatment significantly inhibited the proliferation of HSCs in time- and dose-dependent manners. Loureirin B significantly promoted the apoptosis of HSCs, increased the expression of Bax and decreased the Bcl-2 level. Western blot analysis showed that the expressions of Wnt1 and β-catenin were obviously lower in the loureirin B treatment group than in the control group. We also found that loureirin B could decrease the Wnt1 mRNA level and increase miR-148-3p expression. Knockdown of miR-148-3p using inhibitor could reverse the effects of loureirin B on the proliferation and apoptosis of HSCs and the expressions of Bax, Bcl-2, Wnt1 and β-catenin.ConclusionOur results suggest that loureirin B inhibited the proliferation and promoted the apoptosis of HSCs, and suppressed the Wnt/β-catenin signaling pathway via regulation of miR-148-3p.
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影响因子：
3.5
作者：
Sun, Haitao;Chen, Guanxin;He, Songqi
通讯作者：
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影响因子：
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作者：
Zheng, Jianjian;Wang, Wei;Zhou, Mengtao
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.7
作者：
Qin S;Jiang H;Su S;Wang D;Liang Z;Zhang J;Yang W
通讯作者：
Yang W
影响因子：
13.5
作者：
Yang, Melissa D.;Chiang, Yi-Ming;Higashiyama, Reiichi;Asahina, Kinji;Mann, Derek A.;Mann, Jelena;Wang, Clay C. C.;Tsukamoto, Hidekazu
通讯作者：
Tsukamoto, Hidekazu