Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice
Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice
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水稻抗黑条矮缩病毒病和小褐飞虱数量性状位点鉴定
DOI：
10.1007/s11032-017-0669-x
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发表时间：
2017-06-01
影响因子：
3.1
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Sun, Zhiguang;Liu, Yuqiang;Wan, Jianmin
褐飞虱及其传播的水稻黑条矮缩病（RBSDVD）对水稻造成严重危害。生产虽然抗病品种的选育被认为是RBSDVD管理的最重要的策略之一，很少有高抗线已被发现。在本研究中，我们确定了一个籼稻品种，9194，这是高抗RBSDVD和分析的数量性状位点（QTL），这种抗性。共检测到4个抗RBSDVD的QTL，即qRBSDV 3、qRBSDV 6、qRBSDV 9和qRBSDV 11。其中qRBSDV 6、qRBSDV 9和qRBSDV 11在2年内重复检测，LOD值分别为4.42-4.48、2.11-7.26和5.01-7.16，分别占总表型变异的10.3- 16.7%、8.3- 35.5%和20.0-31.1%。此外，通过标记辅助选择（MAS）将单抗或多抗QTL渗入感病水稻品种中，表明这些QTL的叠加可以逐步增强RBSDVD抗性，表明这些QTL具有加性作用。同一群体也用于SBPH抗性的QTL定位。检测到4个QTL，qSBPH 1、qSBPH 5、qSBPH 8和qSBPH 9，LOD值分别为2.72、2.78、2.15和2.85，分别解释了13.7%、11.0%、12.0%和21.0%的表型变异。本研究通过对水稻抗RBSDVD和SBPH QTL的定位，以及单基因和多基因抗源的开发，为水稻抗RBSDVD和SBPH品种的分子育种提供了新的资源。
Small brown planthopper (SBPH) and its transmitted rice black-streaked dwarf virus disease (RBSDVD) cause serious damage to rice (Oryza sativa L.) production. Though breeding of resistant cultivars is believed to be one of the most important strategies for RBSDVD management, few high-resistance lines have been found to date. In the present study, we identified an indica variety, 9194, that is highly resistant to RBSDVD and analyzed the quantitative trait loci (QTLs) underlying this resistance. In total, four QTLs for RBSDVD resistance, viz. qRBSDV3, qRBSDV6, qRBSDV9, and qRBSDV11, were identified. Among them, qRBSDV6, qRBSDV9, and qRBSDV11 with LOD (logarithm [base 10] of odds) scores of 4.42-4.48, 2.11-7.26, and 5.01-7.16 were repeatedly detected in 2 years, accounting for 10.3-16.7%, 8.3-35.5%, and 20.0-31.1% of the total phenotypic variation, respectively. Further, introgression of single-or multiple-resistance QTLs into a susceptible rice variety by marker-assisted selection (MAS) indicated that stacking the QTLs could progressively enhance RBSDVD resistance, suggesting that these QTLs act additively. The same population was also used for QTL mapping of SBPH resistance. Four QTLs, viz. qSBPH1, qSBPH5, qSBPH8, and qSBPH9, with LOD scores of 2.72, 2.78, 2.15, and 2.85 were detected, explaining 13.7%, 11.0%, 12.0%, and 21.0% of the phenotypic variation, respectively. The identification of RBSDVD and SBPH resistance QTLs, and the development of single and multiple genes with pyramided lines, in this study provides innovative resources for molecular breeding of resistant rice cultivars.