Identification of barosensitive neurons in the mediobasal forebrain using juxtacellular labeling
Identification of barosensitive neurons in the mediobasal forebrain using juxtacellular labeling
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DOI：
10.1152/ajpregu.1999.276.6.r1766
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发表时间：
1999-06-01
影响因子：
2.8
通讯作者：
Pittman, QJ
中科院分区：
文献类型：
--
作者：
Kirouac, GJ;Pittman, QJ
以前的研究表明，在心血管调节的神经元的内侧基底前脑可能的作用。本研究旨在确定基底前脑神经元的位置和形态，对动脉血压的急性变化作出反应。单个单位的细胞外记录是在α-氯醛糖-或β-氯醛糖-麻醉大鼠中进行的。测定了心血管加压（苯肾上腺素，1-2 μ g/kg iv）和降压（硝普钠，0.5-1 μ g/kg iv）事件对单位放电率的影响。随后，一些测试的神经元使用微细胞方法填充生物胞素。使用抗生物素蛋白-生物素复合物反应处理脑切片，以显示神经元的高尔基体样外观。位于Broca斜角带水平支的32个神经元中，有13个（41%）被降压事件兴奋。压力敏感性生物胞素标记的细胞位于hDB的所有区域，并具有小到中等大小的细胞体，具有稀疏和简单的树突状形态。在嗅结节、Calleja岛（IC）和腹侧苍白球区域中测试的47个神经元中只有2个对动脉血压的变化做出反应。本研究的结果表明，在调节心血管功能的hDB神经元的作用。研究结果还表明，嗅结节中的大多数神经元，包括IC复合体，对动脉血压的急性变化没有反应。
Previous investigations suggest a possible role in cardiovascular regulation for neurons of the mediobasal forebrain. The present study was designed to determine the location and morphology of basal forebrain neurons that respond to acute changes in arterial blood pressure. Extracellular recordings of single units were done in alpha-chloralose- or urethan-anesthetized rats. The effect of cardiovascular presser (phenylephrine, 1-2 mu g/kg iv) and depressor (sodium nitroprusside, 0.5-1 mu g/kg iv) events on the discharge rates of units was determined. Some of the neurons tested were subsequently filled with biocytin using the juxtacellular method. Brain sections were processed using the avidin-biotin complex reaction to reveal a Golgi-like appearance of the neuron. Of 32 neurons located in the horizontal limb of the diagonal band of Broca (hDB), 13 (41%) were found to be excited by depressor events. Barosensitive biocytin-labeled cells were located in all regions of the hDB and had small- to medium-sized cell bodies with sparse and simple dendritic morphology. Only 2 of 47 neurons tested in the region of the olfactory tubercle, islands of Calleja (IC), and ventral pallidum responded to changes in arterial blood pressure. The results of the present investigation suggest a role in the regulation of cardiovascular function for neurons of the hDB. The findings also suggest that most neurons in the olfactory tubercle, including the IC complex, do not respond to acute changes in arterial blood pressure.