MEROCYANINE 540 AS AN OPTICAL PROBE OF TRANSMEMBRANE ELECTRICAL-ACTIVITY IN HEART
MEROCYANINE 540 AS AN OPTICAL PROBE OF TRANSMEMBRANE ELECTRICAL-ACTIVITY IN HEART
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DOI：
10.1126/science.191.4226.485
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发表时间：
1976-01-01
期刊：
影响因子：
56.9
通讯作者：
MORAD, M
中科院分区：
文献类型：
--
作者：
SALAMA, G;MORAD, M
用mercury 540染色的青蛙心脏显示，当用540纳米光束激发时，在心脏动作电位期间，585纳米处的荧光强度增加1.5%至2.0%。通过将1毫米的激发束聚焦在心脏的各个区域，记录与起搏器、心房和心室组织中细胞内微电极记录的荧光动作电位相似的荧光动作电位。单个动作电位的信噪比范围为10/1至40/1。在自发起搏的心脏中，荧光动作电位的上升速率较慢是由于电信号的缓慢传播。在含有正常钙浓度的溶液中，荧光信号因收缩而改变。Mercury 540是生物惰性的，因为它染色心脏细胞膜，并作为跨膜电位变化的敏感光学探针。
Frog hearts stained with merocyanine 540 show a 1.5 to 2.0 percent increase in fluorescence intensity at 585 nanometers during the cardiac action potential when excited with a 540-nonometer light beam. Fluorometric action potentials similar to those recorded with intracellular microelectrodes in pacemaker, atrial, and ventricular tissues were recorded by focusing a 1-millimeter excitation beam on various regions of the heart. The signal-to-noise ratio for a single action potential ranged between 10/1 and 40/1. In spontaneously pacing hearts the slower rate of rise of the fluorescence action potential is due to the slow propagation of the electrical signal. In solutions containing normal calcium concentrations the fluorometric signal is altered by contractions. Merocyanine 540 is biologically inert as it stains the cardiac cell membrane and acts as a sensitive optical probe of the change in transmembrane potential.