Aberrant responses to growth-regulatory signals by variant kidney epithelial cells.
Aberrant responses to growth-regulatory signals by variant kidney epithelial cells.
复制标题
变异肾上皮细胞对生长调节信号的异常反应。
DOI：
10.1152/ajprenal.1988.254.5.f747
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Toback,FG
中科院分区：
文献类型：
--
作者：
Walsh-Reitz,MM;Feldman,RI;Toback,FG
在BSC-1系猴肾上皮细胞的生长调节研究期间，观察到细胞密度高于预期的培养物。与亲本细胞相比，当培养物接近汇合时，变异细胞的增殖加速。细胞遗传学分析、特定猴血清的免疫荧光抗体反应、同工酶分析、微生物学研究和软琼脂中生长缺乏表明，变异细胞不是污染细胞类型，缺乏新的同工酶，无微生物污染，且未转化。融合变体培养物对BSC-1细胞产生的纯化生长抑制剂蛋白没有反应，该蛋白抑制亚融合变体和亲本细胞中的增殖并降低细胞Na含量。后叶加压素是亲代细胞的促分裂剂，对变异细胞的融合培养物是一种有效的生长抑制剂。低钾或高钠培养基，刺激亲本细胞的增殖，对变异细胞系的生长没有影响。这些结果表明，变异细胞的增殖增强是由改变的信号转导途径和/或生长调节分子的受体介导的。
Cultures that achieved a higher cell density than expected were noted during study of growth regulation in monkey kidney epithelial cells of the BSC-1 line. Multiplication of the variant cells was accelerated, compared with parental cells, as the cultures approached confluence. Cytogenetic analysis, immunofluorescence antibody reactions with specific monkey serum, isoenzyme analysis, microbiological studies, and lack of growth in soft agar indicated that the variant cells were not a contaminating cell type, lacked new isoenzymes, were free of microbial contamination, and were not transformed. Confluent variant cultures did not respond to a purified growth inhibitor protein produced by BSC-1 cells that inhibits multiplication and reduces cell Na content in subconfluent variant and parental cells. Vasopressin, which is a mitogen for parental cells, was a potent growth inhibitor for confluent cultures of variant cells. Low-K or high-Na media, which stimulate proliferation of parental cells, had no effect on growth of the variant cell line. These results suggest that enhanced multiplication of the variant cells is mediated by altered signal transduction pathways and/or receptors for growth-regulatory molecules.