Differences in development of bovine oocytes recovered by aspiration or by mincing.
Differences in development of bovine oocytes recovered by aspiration or by mincing.
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通过抽吸或切碎回收的牛卵母细胞发育差异。
DOI：
10.2527/1992.7061923x
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发表时间：
1992
影响因子：
3.3
通讯作者：
J. Masaki
中科院分区：
文献类型：
--
作者：
Y. Takagi;K. Mori;T. Takahashi;S. Sugawara;J. Masaki
本试验旨在研究牛卵巢状况与卵泡卵母细胞发育能力之间的关系，并比较两种卵母细胞采集方法，即抽吸法和切碎法。从24对卵巢中抽取卵泡或切碎组织，回收周围有完整未扩张卵丘的卵泡卵母细胞，使其体外成熟和受精。从成对卵巢中回收的卵泡卵母细胞的数量平均为32.1 +/- 3.2，但24对卵巢中回收的数量差异很大（范围，7至71）。发育到囊胚阶段的总体比率为18%（137/771），从一对卵巢产生的平均囊胚数为5.7 +/- 1.1（范围，0至17）。没有发现黄体或大卵泡的存在与体外能够达到囊胚阶段的卵母细胞的比例之间的关系。然而，从每对卵巢中获得的卵母细胞数量与随后的体外发育之间存在正相关性;通过抽吸获得的卵母细胞的相关性尤其高。这些数据表明，牛卵泡卵母细胞体外成熟和受精后的发育能力与从一对卵巢中吸出的有腔卵泡数量相关。
This experiment was performed to clarify relationships between conditions of bovine ovaries and developmental capacity of the follicular oocytes recovered from them and to compare two methods of oocyte collection, aspiration and mincing. Follicular oocytes with surrounding intact, unexpanded cumulus recovered by follicular aspiration or by mincing of tissue from 24 pairs of ovaries were matured and fertilized in vitro. The number of follicular oocytes recovered from pairs of ovaries averaged 32.1 +/- 3.2, but the number recovered varied greatly among the 24 pairs of ovaries (range, 7 to 71). The overall rate of development to the blastocyst stage was 18% (137/771), and the average number of blastocysts produced from a pair of ovaries was 5.7 +/- 1.1 (range, 0 to 17). No relationships were found between the presence of corpora lutea or large follicles and the proportion of oocytes capable of reaching the blastocyst stage in vitro. However, a positive correlation was observed between the number of oocytes obtained from each pair of ovaries and subsequent in vitro development; the correlation was especially high for oocytes obtained by aspiration. These data suggest that the developmental capacity of bovine follicular oocytes after in vitro maturation and fertilization is correlated to the number of antral follicles aspirated from the pair of ovaries.