Type 1, 2, and 1/2-Hybrid IncC Plasmids From China
Type 1, 2, and 1/2-Hybrid IncC Plasmids From China
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DOI：
10.3389/fmicb.2019.02508
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发表时间：
2019-11-15
影响因子：
5.2
通讯作者：
Dai, Erhei
中科院分区：
文献类型：
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作者：
Cheng, Qiaoxiang;Jiang, Xiaoyuan;Dai, Erhei
本文对来自中国的11个IncC质粒的集合进行了全测序，并与参考质粒pR 148和pR 55进行了比较。这13个质粒可分为3个不同的亚组：1型、2型和1/2型杂合体。1/2型杂合质粒最有可能是由1型和2型质粒之间的同源重组产生的。不同的IncC质粒已经进化到获得完全不同的辅助模块，从而获得不同的抗性基因集合。辅助耐药模块不仅包括bla（CMY）携带区、ARI-A岛和ARI-B岛，还包括bla（CTX-M）携带区和MDR区等各种附加类型的耐药岛。附件模块的插入有时伴随着周围骨架区域的缺失、倒位和易位。pR 148和pR 55被证实分别具有1型和2型的最完整骨架。这是首次报道携带bla（IMP-）（8）的IncC质粒，以及三个新的移动的元件：Tn 1696衍生的单位转座子Tn 6395、2类整合子In 2 -76和插入序列ISEcl 10。
A collection of 11 IncC plasmids from China were fully sequenced herein and compared with reference plasmids pR148 and pR55. These 13 plasmids could be assigned into three different subgroups: type 1, type 2, and type 1/2 hybrid. Type 1/2-hybrid plasmids most likely emerged from homologous recombination between type 1 and type 2 plasmids. Different IncC plasmids had evolved to acquire quite different profiles of accessory modules and thus different collections of resistance genes. The accessory resistance modules included not only the bla(CMY)-carrying region, the ARI-A island, and the ARI-B island, but also various additional kinds of resistance islands such as the bla(CTX-M)-carrying regions and the MDR regions. Insertion of accessory modules was sometimes accompanied by deletion, inversion, and translocation of surrounding backbone regions. pR148 and pR55 were confirmed to have the most complete backbones for type 1 and type 2, respectively. This was the first report of a bla(IMP-)(8)-carrying IncC plasmid, and that of three novel mobile elements: a Tn1696-derived unit transposon Tn6395, a class 2 integron In2-76, and an insertion sequence ISEcl10.