Physiological effects of ultrasound mist on fibroblasts
Physiological effects of ultrasound mist on fibroblasts
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DOI：
10.1111/j.1365-4632.2007.02914.x
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发表时间：
2007-06-01
影响因子：
3.6
通讯作者：
Pittelkow, Mark R.
中科院分区：
文献类型：
--
作者：
Lai, Jengyu;Pittelkow, Mark R.
背景慢性伤口在医疗保健中提出了越来越多的挑战，并且消耗了医疗保健成本的很大一部分。虽然已经开发了新的治疗方法，但治疗成功率并没有得到很大提高。超声波在医学上应用已久。其独特的能量输送能力使其成为伤口护理模式的理想候选者。我们提出超声对细胞内信号通路的影响是不同的，并利用非接触式超声评估这种影响的能力，提供了一种测试这种建议的方法。研究了超声处理后真皮成纤维细胞转化生长因子β 1（TGF-β 1）、细胞外调节激酶（ERK）和c-Jun N-末端激酶（JNK）信号通路的激活。未处理和刮伤成纤维细胞作为controls.Results没有观察到的形态学差异，除了在超声处理的成纤维细胞空泡化。有丝分裂活性之间的超声处理和刮伤成纤维细胞相似。超声处理的成纤维细胞表现出更早的KGF表达，ERK激活，JNK激活的增加。结论超声波可诱导成纤维细胞的细胞反应，这可能有利于创伤愈合。
Background Chronic wounds present an increasing challenge in healthcare and consume a substantial portion of healthcare cost. Although new treatments have been developed, treatment success has not been improved greatly. Ultrasound has long been employed in medicine. Its unique ability to deliver energy makes it an ideal candidate as a wound care modality. We proposed that ultrasound would differentially affect intracellular signaling pathways and, with the ability to assess this effect using a noncontact form of ultrasound, were provided with a means to test this proposal.Methods The cellular morphology, mitogenic activities, expression of keratinocyte growth factor (KGF) and transforming growth factor beta-1 (TGF-beta 1), and activation of extracellular regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways of dermal fibroblasts were studied after ultrasound treatment. Untreated and scrape-wounded fibroblasts were utilized as controls.Results There was no difference in morphology observed, except for vacuolization in ultrasound-treated fibroblasts. Mitogenic activities were similar between ultrasound-treated and scrape-wounded fibroblasts. Ultrasound-treated fibroblasts exhibited a much earlier increase in KGF expression, ERK activation, and JNK activation. The ERK/JNK ratio was increased markedly in ultrasound-treated fibroblasts.Conclusion We conclude that ultrasound induces cellular responses that may be beneficial to wound healing.