Effects of cryoprotectants on viability of Lactobacillus reuteri CICC6226
Effects of cryoprotectants on viability of Lactobacillus reuteri CICC6226
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冷冻保护剂对罗伊氏乳杆菌 CICC6226 活力的影响
DOI：
10.1007/s00253-011-3269-4
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发表时间：
2011-11-01
影响因子：
5
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Li, Baokun;Tian, Fengwei;Chen, Wei
冷冻干燥通常用于保存益生菌，但它可能导致细胞损伤和活力丧失。冷冻保护剂在冷冻干燥过程中的活力保护中起着重要作用。在这项研究中，我们研究了在冷冻保护剂，如蔗糖，海藻糖，和重构脱脂乳（RSM）的存在下，罗伊氏乳杆菌CICC 6226的存活率。此外，我们测定了活性的己糖激酶（HK），丙酮酸激酶（PK），乳酸脱氢酶（LDH），和ATP酶冷冻干燥后立即。结果表明，在冻干过程中，加入保护剂前后HK和PK活性差异不显著，但冻干前后细胞活力、LDH和ATPase活性差异极显著（P<0.01）。结果表明，添加10%海藻糖和10%丹参，可提高冻干过程中膜的完整性和流动性。这些结果为冷冻干燥过程中细胞损伤提供了直接的生化和代谢证据。冷冻干燥破坏了细胞膜结构和功能，使LDH和ATP酶失活。结果表明，乳酸脱氢酶和三磷酸腺苷酶是评价冷冻保护剂对乳酸杆菌存活率和代谢活性影响的重要指标。reuteri CICC6226在冷冻干燥过程中。
Freeze-drying is commonly used to preserve probiotics, but it could cause cell damage and loss of viability. The cryoprotectants play an important role in the conservation of viability during freeze-drying. In this study, we investigated the survival rates of Lactobacillus reuteri CICC6226 in the presence of cryoprotectants such as sucrose, trehalose, and reconstituted skim milk (RSM). In addition, we determined the activities of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and ATPases immediately following the freeze-drying. The results showed that the differences in HK and PK activities with and without the cryoprotectants during freeze-drying were not significant, but cell viability and activities of LDH and ATPase were significantly different (P<0.01) prior to and after freeze-drying. Meanwhile, the results showed that the maintenance of the membrane integrity and fluidity was improved in the presence of the 10% trehalose or 10% RSM than other treatments during freeze-drying. These results have provided direct biochemical and metabolic evidence of injured cell during freeze-drying. Freeze-drying damaged membrane structure and function of cell and inactivated enzymes (LDH and ATPases). The results imply that LDH and ATPases are key markers and could be used to evaluate the effect of cryoprotectants on viability and metabolic activities of L. reuteri CICC6226 during freeze-drying.