Dpp gradient formation in the Drosophila wing imaginal disc
Dpp gradient formation in the Drosophila wing imaginal disc
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DOI：
10.1016/s0092-8674(00)00199-9
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发表时间：
2000-12-08
期刊：
影响因子：
64.5
通讯作者：
Cohen, SM
中科院分区：
文献类型：
--
作者：
Teleman, AA;Cohen, SM
分泌的信号蛋白 Dpp 作为形态发生素来塑造果蝇翅膀的前后轴。发育中的翼成虫盘的所有细胞都需要 Dpp 活性，但支持这种活性的配体梯度尚未被表征。在这里，我们利用带有 GFP 标记的 Dpp 生物活性形式来检查配体梯度。 Dpp-GFP 形成不稳定的细胞外梯度，在翼盘中快速扩散。 MAD 磷酸化显示的活性梯度在形状上与配体梯度不同。当通过实验改变时，pMAD 梯度根据隔室大小进行调整。这些观察结果表明 Dpp 活性梯度可能在受体激活水平上形成。
The secreted signaling protein Dpp acts as a morphogen to pattern the anterior-posterior axis of the Drosophila wing. Dpp activity is required in all cells of the developing wing imaginal disc, but the ligand gradient that supports this activity has not been characterized. Here we make use of a biologically active form of Dpp tagged with GFP to examine the ligand gradient. Dpp-GFP forms an unstable extracellular gradient that spreads rapidly in the wing disc. The activity gradient visualized by MAD phosphorylation differs in shape from the ligand gradient. The pMAD gradient adjusted to compartment size when this was experimentally altered. These observations suggest that the Dpp activity gradient may be shaped at the level of receptor activation.