Metagenomic analysis of a high carbon dioxide subsurface microbial community populated by chemolithoautotrophs and bacteria and archaea from candidate phyla
Metagenomic analysis of a high carbon dioxide subsurface microbial community populated by chemolithoautotrophs and bacteria and archaea from candidate phyla
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DOI：
10.1111/1462-2920.12817
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发表时间：
2016-06-01
影响因子：
5.1
通讯作者：
Banfield, Jillian F.
中科院分区：
文献类型：
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作者：
Emerson, Joanne B.;Thomas, Brian C.;Banfield, Jillian F.
地质固碳研究提出了地下微生物群对碳循环和生物地球化学的潜在影响的问题。地下高二氧化碳系统的生物学特征很差，部分原因是难以获取大量未受污染的样品。 CO2 驱动的水晶间歇泉（CG，犹他州，美国）是一种成熟的地质碳封存类似物，可提供大量深层（类似于 200-500 m）地下流体。我们通过组装和分析从间歇泉水滤液中回收的宏基因组，探索了这种高二氧化碳环境中的微生物多样性和代谢潜力。该系统以中性粒细胞、铁氧化细菌为主，包括“海洋”Mariprofundus（Zetaproteobacteria）和“淡水”Gallionellales、硫氧化Thiomicrospira crunogena 和类硫杆菌属嗜氢菌。这些细菌的基因组近乎完整。 CG 中明显存在着多种细菌和古细菌，这些细菌和古细菌来自缺乏分离代表的门（候选门）和来自尚未定义的谱系。许多细菌隶属于 OD1、OP3、OP9、PER、ACD58、WWE3、BD1-5、OP11、TM7 和 ZB2。近 100 个编码卡尔文循环和 AMP 补救途径的核酮糖-1,5 二磷酸羧化酶加氧酶亚基蛋白的基因的恢复表明在高 CO2 地下碳循环中具有强大的生物学作用。总体而言，我们预测微生物通过铁、硫和复杂的碳氧化、碳和氮固定、发酵、氢代谢以及有氧和无氧呼吸对地下生物地球化学的影响。
Research on geologic carbon sequestration raises questions about potential impacts of subsurface microbiota on carbon cycling and biogeochemistry. Subsurface, high-CO2 systems are poorly biologically characterized, partly because of difficulty accessing high-volume, uncontaminated samples. CO2-driven Crystal Geyser (CG, Utah, USA), an established geologic carbon sequestration analogue, provides high volumes of deep (similar to 200-500 m) subsurface fluids. We explored microbial diversity and metabolic potential in this high-CO2 environment by assembly and analysis of metagenomes recovered from geyser water filtrate. The system is dominated by neutrophilic, iron-oxidizing bacteria, including 'marine' Mariprofundus (Zetaproteobacteria) and 'freshwater' Gallionellales, sulfur-oxidizing Thiomicrospira crunogena and Thiobacillus-like Hydrogenophilales. Near-complete genomes were reconstructed for these bacteria. CG is notably populated by a wide diversity of bacteria and archaea from phyla lacking isolated representatives (candidate phyla) and from as-yet undefined lineages. Many bacteria affiliate with OD1, OP3, OP9, PER, ACD58, WWE3, BD1-5, OP11, TM7 and ZB2. The recovery of nearly 100 genes encoding ribulose-1,5 bisphosphate carboxylase-oxygenase subunit proteins of the Calvin cycle and AMP salvage pathways suggests a strong biological role in high-CO2 subsurface carbon cycling. Overall, we predict microbial impacts on subsurface biogeochemistry via iron, sulfur, and complex carbon oxidation, carbon and nitrogen fixation, fermentation, hydrogen metabolism, and aerobic and anaerobic respiration.