Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells
Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells
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DOI：
10.1387/ijdb.041948tj
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发表时间：
2005-01-01
影响因子：
0.7
通讯作者：
Drevon, C
中科院分区：
文献类型：
--
作者：
Jaffredo, T;Bollerot, K;Drevon, C
我们在这里回顾造血系统的发展及其与内皮的关系，特别关注血管母细胞的特征，血管母细胞是内皮细胞和造血细胞的假定祖先。使用禽类模型，我们已经在体内追踪了胚胎内皮细胞的后代，并显示出造血干细胞（已知产生确定的造血谱系）来源于主动脉底部的内皮细胞。在此过程中，内皮细胞经历从内皮细胞到造血细胞的转换，其特征在于内皮细胞特异性基因的降级和造血细胞特异性基因的平行升级。使用类似的方法，我们已经表明，从内皮细胞生成造血细胞也发生在小鼠胚胎发育过程中。我们已经彻底的特点动态的关键分子（其中几个我们已经克隆）特异性表达的卵黄囊或主动脉成血管细胞。卵黄囊成血管细胞的特征在于SCL/Tal-1和Lmo 2的特异性表达，而主动脉成血管细胞表达Runx-1（一种runt结构域转录因子）。最后，我们证明了一个新的造血部位，即尿囊的存在。使用鹌鹑/鸡移植物，我们表明，这个胚胎附肢自主产生内皮细胞和造血细胞，这些后者被赋予的属性的最终造血谱系。
We review here the development of the hematopoietic system and its relationship to the endothelium, with a special focus on the characterisation of the hemangioblast, the putative ancestor for endothelial cells and hematopoietic cells. Using the avian model, we have traced in vivo the progeny of embryonic endothelial cells and shown that aortic-born hematopoietic cells (known to generate the definitive hematopoietic lineage) derive from endothelial cells in the floor of the aorta. During this process, endothelial cells undergo a switch from endothelial cells to hematopoietic cells characterised by a downgrading of endothelial cell-specific genes and the parallel upgrading of hematopoietic cell-specific genes. Using a similar approach, we have shown that generation of hematopoietic cells from endothelial cells also takes place during mouse embryonic development. We have thoroughly characterised the dynamics of key molecules (several of which we have cloned) specifically expressed by the yolk sac or aortic hemangioblast. The yolk sac hemangioblast is characterized by the specific expression of SCL/Tal-1 and Lmo2, whereas the aortic hemangioblast expresses Runx-1 (a runt domain transcription factor). Finally, we have demonstrated the existence of a new site for hematopoiesis, namely the allantois. Using quail/chick grafts, we show that this embryonic appendage autonomously produces endothelial cells and hematopoletic cells, these latter being endowed with the attributes of the definitive hematopoietic lineage.