Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice
Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice
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DOI：
10.1105/tpc.105.035899
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发表时间：
2005-12-01
期刊：
影响因子：
11.6
通讯作者：
Shinomura, T
中科院分区：
文献类型：
--
作者：
Takano, M;Inagaki, N;Shinomura, T
我们已经从水稻中分离出光敏色素B（phyB）和phyC突变体，并产生了所有的双突变体组合。phyB和phyB phyC突变体的幼苗表现出对连续红光（Rc）的敏感性部分丧失，但仍表现出显着的脱黄化反应。在phyA和phyB双突变体中，Rc的反应被完全取消。这些结果表明phyA和phyB以高度冗余的方式控制Rc下的脱黄化。在连续远红光（FRc）下，phyA突变体表现出部分受损的脱黄化，phyA phyC双突变体表现出不明显的残留光敏色素反应，表明不仅phyA而且phyC参与水稻FRc的光感受。有趣的是，phyB phyC双突变体在脉冲辐照实验中显示出明显的R/FR可逆性，表明phyA和phyB都可以介导基因表达的低通量响应。水稻是一种短日照植物，在长日照条件下，phyB或phyC基因突变可导致水稻中度早花，而phyA基因突变对水稻的开花时间影响不大。然而，phyA突变与phyB或phyC突变的组合导致显着的早花。
We have isolated phytochrome B (phyB) and phyC mutants from rice (Oryza sativa) and have produced all combinations of double mutants. Seedlings of phyB and phyB phyC mutants exhibited a partial loss of sensitivity to continuous red light (Rc) but still showed significant deetiolation responses. The responses to Rc were completely canceled in phyA phyB double mutants. These results indicate that phyA and phyB act in a highly redundant manner to control deetiolation under Rc. Under continuous far-red light (FRc), phyA mutants showed partially impaired deetiolation, and phyA phyC double mutants showed no significant residual phytochrome responses, indicating that not only phyA but also phyC is involved in the photoperception of FRc in rice. Interestingly, the phyB phyC double mutant displayed clear R/FR reversibility in the pulse irradiation experiments, indicating that both phyA and phyB can mediate the low-fluence response for gene expression. Rice is a short-day plant, and we found that mutation in either phyB or phyC caused moderate early flowering under the long-day photoperiod, while monogenic phyA mutation had little effect on the flowering time. The phyA mutation, however, in combination with phyB or phyC mutation caused dramatic early flowering.