Characterization of transmembrane chemical shift differences in the phosphorus-31 NMR spectra of various phosphoryl compounds added to erythrocyte suspensions
Characterization of transmembrane chemical shift differences in the phosphorus-31 NMR spectra of various phosphoryl compounds added to erythrocyte suspensions
复制标题
添加到红细胞悬浮液中的各种磷酰基化合物的磷 31 NMR 谱中跨膜化学位移差异的表征
DOI：
10.1021/bi00424a016
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
P. Kuchel
中科院分区：
文献类型：
--
作者：
K. Kirk;P. Kuchel
磷酸三甲酯、甲基膦酸二甲酯、甲基膦酸二乙酯、氧化三甲基膦以及次磷酸盐、苯基膦酸和二苯基膦酸离子都含有磷基官能团。当添加到完整的红细胞悬浮液中时，每种化合物在20/度/C下产生分离的细胞内和细胞外/sup 31/P NMR共振，并且每种化合物的两个共振之间的分离随平均细胞体积而变化。细胞内和细胞外化学变化的差异主要归因于血红蛋白的作用。细胞内血红蛋白的存在导致了两个隔室磁化率的显著差异。此外，它还具有很大的与敏感性无关的化学位移效应，其大小取决于所涉及的磷酰化合物的化学结构。许多其他细胞内和细胞外成分也被证明会引起化学位移变化，比血红蛋白引起的变化小，但仍然很重要。因此，跨膜化学位移差异的细胞体积依赖性不仅反映了细胞内血红蛋白浓度的细胞体积依赖性，还反映了两个室中其他溶质浓度的变化。除了对细胞体积的依赖性外，跨膜化学位移差异也随着温度的变化而变化。在非电解质的情况下，这不仅反映了负责敏感性无关位移效应的机制的温度依赖性，而且反映了化合物穿过细胞膜的速率的温度依赖性。«少
Trimethyl phosphate, dimethyl methylphosphonate, diethyl methylphosphonate, trimethylphosphine oxide, and the hypophosphite, phenylphosphinate, and diphenylphosphinate ions all contain the phosphoryl functional group. When added to an intact erythrocyte suspension at 20/degrees/C, each of the compounds gave rise to separate intra- and extracellular /sup 31/P NMR resonances, and the separation between the two resonances of each compound varied with the mean cell volume. The differences between the intra- and extracellular chemical shifts were shown to be primarily attributable to the effects of hemoglobin. The presence of hemoglobin inside the cell gave rise to a significant difference in the magnetic susceptibilities of the two compartments. In addition, it exerted a large susceptibility-independent chemical shift effect, the magnitude of which was dependent upon the chemical structure of the phosphoryl compound involved. A number of other intra- and extracellular components were also shown to cause chemical shift variations, smaller than those arising from hemoglobin but nonetheless significant. The cell volume dependence of the transmembrane chemical shift differences therefore reflected not only the cell volume dependence of the intracellular hemoglobin concentration but also the changing concentration of the other solutes in the two compartments. In addition to their cell volume dependence, the transmembrane chemical shift differencesmore » varied with temperature. In the case of the nonelectrolytes this reflected not only the temperature dependence of the mechanism(s) responsible for the susceptibility-independent shift effects but also the temperature dependence of the rates at which the compounds traversed the cell membrane.« less
磷酸盐类似物的红细胞阴离子转运。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Labotka,RJ;Omachi,A
通讯作者：
Omachi,A
影响因子：
2.9
作者：
FABRY, ME;GEORGE, RCS
通讯作者：
GEORGE, RCS
通过核磁共振波谱研究可滴定阴离子的红细胞膜转运的 pH 依赖性。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Labotka,RJ;Omachi,A
通讯作者：
Omachi,A