The poor osteoinductive capability of human acellular bone matrix.
The poor osteoinductive capability of human acellular bone matrix.
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DOI：
10.5301/ijao.5000122
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
Yizhou Huang;Jiaqin Cai;Jing Xue;Xiao-he Chen;Chao-Liang Zhang;Xiu-qun Li;Zhi-ming Yang;
中科院分区：
文献类型：
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作者：
Yizhou Huang;Jiaqin Cai;Jing Xue;Xiao-he Chen;Chao-Liang Zhang;Xiu-qun Li;Zhi-ming Yang;
脱矿骨基质（DBM）由于其骨诱导和骨传导能力而在临床上广泛用于骨再生。这表明，在骨基质制备的脱矿过程是有影响的，在保持骨诱导性，然而，相关的调查，特别是脱细胞骨基质的骨诱导性，往往没有进行。本研究探讨了人脱细胞松质骨基质（ACBM）皮下植入大鼠模型后的骨诱导能力。同时观察大鼠骨髓间充质干细胞（rBM-MSCs）在该材料中的生长和成骨分化情况。在没有脱矿过程的情况下，我们获得的ACBM具有互连的多孔网络，并且表面的微孔清晰地暴露出来。ACBM皮下植入4个月后，观察到新的类骨质形成，但不是典型的成熟骨形成。rBM-MSCs在ACBM中生长良好，连续培养28 d后形态稳定。然而，未检测到矿化结节形成，编码成骨标志物的基因的表达水平显著降低。这些结果表明，人ACBM具有天然骨的结构特征和较差的骨诱导性;尽管如此，这种材料有助于保持rBM-MSCs的未分化表型。这些见解可能会进一步拓宽我们对ACBM在骨再生和干细胞壁龛创建中的应用的理解。
Demineralized bone matrix (DBM) has extensive clinical use for bone regeneration because of its osteoinductive and osteoconductive aptitude. It is suggested that the demineralization process in bone matrix preparation is influential in maintaining osteoinductivity; however, relevant investigations, especially into the osteoinductivity of acellular bone matrix, are not often performed. This study addressed the osteoinductive capability of human acellular cancellous bone matrix (ACBM) after subcutaneous implantation in a rat model. The growth and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) seeded in this material were also studied. Without the demineralization process, the ACBM we obtained had an interconnected porous network and the micropores in the surface were clearly exposed. After the ACBM was subcutaneously implanted for 4 months, new osteoid formation was noted but not typical mature bone formation. rBM-MSCs grew well in the ACBM and kept a steady morphology after continuous culture for 28 days. However, no mineralized nodule formation was detected and the expression levels of genes encoding osteogenic markers were significantly decreased. These results demonstrated that human ACBM possess the structural features of native bone and poor osteoinductivity; nonetheless this material helped to preserve the undifferentiated phenotype of rBM-MSCs. Such insights may further broaden our understanding of the application of ACBM for bone regeneration and the creation of stem cell niches.