Tracing the expression of circular RNAs in human pre-implantation embryos.
Tracing the expression of circular RNAs in human pre-implantation embryos.
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追踪人类植入前胚胎中环状 RNA 的表达
DOI：
10.1186/s13059-016-0991-3
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发表时间：
2016-06-17
期刊：
影响因子：
12.3
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Dang Y;Yan L;Hu B;Fan X;Ren Y;Li R;Lian Y;Yan J;Li Q;Zhang Y;Li M;Ren X;Huang J;Wu Y;Liu P;Wen L;Zhang C;Huang Y;Tang F;Qiao J
由于材料的缺乏，PolyA - rna尚未在人类胚胎植入前广泛分析。特别是环状RNA (circRNA)，一种新型的多a - RNA，在人类植入前发育过程中尚未被表征。我们使用SUPeR-seq系统地分析了个体人类卵母细胞和着床前胚胎中的polyA+信使rna （mrna）和polyA - rna。我们从2974个宿主基因中重新鉴定出10032个环状rna。这些circrna大多是发育阶段特异性和动态调控的。它们中的许多是母系表达的，这意味着它们在卵子发生和全能性合子的形成中具有潜在的重要调节功能。人类和小鼠胚胎的比较表明，这两个物种具有高度的保守性和明显的区别。与小鼠胚胎相比，人类植入前胚胎产生更多类型的circRNA，这与人类内含子侧翼circRNA长度的显著增加有关。我们还进行RNA从头组装和鉴定新的转录单位，其中许多是潜在的新的长链非编码RNA。本研究首次报道了人类植入前发育过程中包含polyA+ mrna和polyA - rna（包括circRNAs）的整个转录组的分析。它为分析这一过程中circrna的独特功能和复杂调控机制提供了宝贵的资源。本文的在线版本（doi:10.1186/s13059-016-0991-3）包含补充材料，可供授权用户使用。
PolyA– RNAs have not been widely analyzed in human pre-implantation embryos due to the scarcity of materials. In particular, circular RNA (circRNA), a novel type of polyA– RNA, has not been characterized during human pre-implantation development. We systematically analyze polyA+ messenger RNAs (mRNAs) and polyA– RNAs in individual human oocytes and pre-implantation embryos using SUPeR-seq. We de novo identify 10,032 circRNAs from 2974 hosting genes. Most of these circRNAs are developmentally stage-specific and dynamically regulated. Many of them are maternally expressed, implying their potentially important regulatory functions in oogenesis and the formation of totipotent zygotes. Comparison between human and mouse embryos reveals both high conservation and clear distinction between these two species. Human pre-implantation embryos generate more types of circRNA compared with mouse embryos and this is associated with a striking increase of the length of the circRNA flanking introns in humans. We also perform RNA de novo assembly and identify novel transcript units, many of which are potentially novel long non-coding RNAs. This study reports the first analysis of the whole transcriptome comprising both polyA+ mRNAs and polyA– RNAs including circRNAs during human pre-implantation development. It provides an invaluable resource for analyzing the unique function and complex regulatory mechanisms of circRNAs during this process. The online version of this article (doi:10.1186/s13059-016-0991-3) contains supplementary material, which is available to authorized users.