P-glycoprotein on astrocyte foot processes of unfixed isolated human brain capillaries
P-glycoprotein on astrocyte foot processes of unfixed isolated human brain capillaries
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DOI：
10.1016/s0006-8993(98)01305-5
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发表时间：
1999-02-20
期刊：
影响因子：
2.9
通讯作者：
Pardridge, WM
中科院分区：
文献类型：
--
作者：
Golden, PL;Pardridge, WM
Sites of immunoreactive P-glycoprotein associated with human brain microvasculature were identified by labeling of unfixed isolated human brain capillaries, allowing visualization of the three-dimensional capillary structure by confocal microscopy. Capillaries isolated from human autopsy brain were dual-labeled with the MRK16 mouse monoclonal antibody (against human P-glycoprotein) and rabbit polyclonal antisera against the human brain microvascular glucose transporter (GLUT1), or glial fibrillary acidic protein (GFAP) on astrocyte foot processes. MRK16 and GLUT1 dual-labeling showed no signal overlap, identical to the staining pattern observed for dual-labeling with anti-GFAP and anti-GLUT1 antibodies: both GFAP and MRK16 labeling were discrete, discontinuous, and not co-localized with continuous GLUT1 labeling of capillary endothelium. In contrast, complete overlap of MRK16 and GFAP labeling demonstrated P-glycoprotein localization on astrocyte foot process remnants at the abluminal face of the brain microvasculature. (C) 1999 Elsevier Science B.V. All rights reserved.
