Methylglyoxal and other carbohydrate metabolites induce lanthanum-sensitive Ca2+ transients and inhibit growth in E-coli
Methylglyoxal and other carbohydrate metabolites induce lanthanum-sensitive Ca2+ transients and inhibit growth in E-coli
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DOI：
10.1016/j.abb.2007.09.006
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发表时间：
2007-12-01
影响因子：
3.9
通讯作者：
Wann, Kenneth T.
中科院分区：
文献类型：
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作者：
Campbell, Anthony K.;Naseern, Riffat;Wann, Kenneth T.
这里的结果首次证明了一系列碳水化合物发酵产品在细菌中打开 Ca2+ 通道。甲基乙二醛、乙偶姻（乙酰甲基甲醇）、二乙酰（2,3 丁二酮）和丁烷 2,3 二醇在大肠杆菌中诱导 Ca2+ 瞬变，由水母发光蛋白监测，显然是通过打开 Ca2+ 通道。甲基乙二醛最有效（K-1/2=1 mM，对于丁烷 2,3 二醇为 50 mM）。 Ca2+ 瞬变取决于外部 Ca2+ (0.1-10 mM)，并被 La3+ (5 mM) 阻断。代谢物影响生长，其中甲基乙二醛最为有效，可完全阻止生长长达 5 小时而不杀死细胞。但24小时后对活细胞数量没有影响。这些结果与激活 La3+ 敏感 Ca2+ 通道的碳水化合物产品一致，胞质 Ca2+ 的增加可能可以防止某些毒素。它们对细菌-宿主细胞信号传导以及大量不同细菌竞争相同底物（例如乳糖和食物不耐受的肠道）具有重要意义。 (C) 2007 Elsevier Inc. 保留所有权利。
The results here are the first demonstration of a family of carbohydrate fermentation products opening Ca2+ channels in bacteria. Methylglyoxal, acetoin (acetyl methyl carbinol), diacetyl (2,3 butane dione), and butane 2,3 diol induced Ca2+ transients in Escherichia coli, monitored by aequorin, apparently by opening Ca2+ channels. Methylglyoxal was most potent (K-1/2=1 mM, 50 mM for butane 2,3 diol). Ca2+ transients depended on external Ca2+ (0.1-10 mM), and were blocked by La3+ (5 mM). The metabolites affected growth, methylglyoxal being most potent, blocking growth completely up to 5 h without killing the cells. But there was no affect on the number of viable cells after 24 h. These results were consistent with carbohydrate products activating a La3+-sensitive Ca2+ channel, rises in cytosolic Ca2+ possibly protecting against certain toxins. They have important implications in bacterial-host cell signalling, and where numbers of different bacteria compete for the same substrates, e.g., the gut in lactose and food intolerance. (C) 2007 Elsevier Inc. All rights reserved.