Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami.
Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami.
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DOI：
10.7554/elife.76357
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发表时间：
2022-06-24
期刊：
影响因子：
7.7
通讯作者：
Carter, Andrew P.
中科院分区：
文献类型：
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作者：
Abdellatef, Shimaa A.;Tadakuma, Hisashi;Yan, Kangmin;Fujiwara, Takashi;Fukumoto, Kodai;Kondo, Yuichi;Takazaki, Hiroko;Boudria, Rofia;Yasunaga, Takuo;Higuchi, Hideo;Hirose, Keiko;Carter, Andrew P.
当轴突一侧的轴突动力蛋白分子产生力并向微管（MT）负端移动时，纤毛和鞭毛发生弯曲。当轴突向另一个方向弯曲时，这些动力蛋白被拉回，这意味着在纤毛/鞭毛的重复弯曲过程中，动力蛋白来回振荡运动。有各种各样的因素可以调节动力蛋白的活性，例如，连接蛋白-动力蛋白调节复合体、径向辐条和中央装置。为了了解动力蛋白振荡运动的基本机制，我们构建了一个由MTs、外臂动力蛋白以及由DNA折纸制成的MTs之间的交联组成的简单模型系统。电子显微镜（EM）显示了由排列规则的动力蛋白分子斑块以两种不同方向结合的平行mt对交叉桥接，这取决于它们的尾巴与哪个mt结合。当一对mt具有相同的极性时，相反取向的动力预计会产生相反的力。光学捕获实验表明，dynein-MT-DNA-origami复合物在被囚禁的ATP光解后实际上是来回振荡的。有趣的是，当握住复合体的一端时，它会表现出重复的弯曲运动。结果表明，一个由相反取向的动力系统、mt和mt间交联剂组成的简单系统，没有任何额外的调节结构，具有引起振荡和重复弯曲运动的内在能力。
Bending of cilia and flagella occurs when axonemal dynein molecules on one side of the axoneme produce force and move toward the microtubule (MT) minus end. These dyneins are then pulled back when the axoneme bends in the other direction, meaning oscillatory back and forth movement of dynein during repetitive bending of cilia/flagella. There are various factors that may regulate the dynein activity, e.g. the nexin-dynein regulatory complex, radial spokes, and central apparatus. In order to understand the basic mechanism of dynein’s oscillatory movement, we constructed a simple model system composed of MTs, outer-arm dyneins, and crosslinks between the MTs made of DNA origami. Electron microscopy (EM) showed pairs of parallel MTs crossbridged by patches of regularly arranged dynein molecules bound in two different orientations, depending on which of the MTs their tails bind to. The oppositely oriented dyneins are expected to produce opposing forces when the pair of MTs have the same polarity. Optical trapping experiments showed that the dynein-MT-DNA-origami complex actually oscillates back and forth after photolysis of caged ATP. Intriguingly, the complex, when held at one end, showed repetitive bending motions. The results show that a simple system composed of ensembles of oppositely oriented dyneins, MTs, and inter-MT crosslinkers, without any additional regulatory structures, has an intrinsic ability to cause oscillation and repetitive bending motions.
DOI：
10.1083/jcb.101.4.1400
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sale WS;Goodenough UW;Heuser JE
通讯作者：
Heuser JE