17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells
17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells
复制标题
17β-雌二醇和黄体酮降低 MDP 诱导的牛乳腺上皮细胞中 NOD2 的表达
DOI：
10.1016/j.vetimm.2017.04.010
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发表时间：
2017-06-01
影响因子：
1.8
通讯作者：
Wu, Rui
中科院分区：
文献类型：
--
作者：
Xu, Dan-dan;Wang, Gang;Wu, Rui
During the periparturient period, many neuroendocrine changes develop in cows. Periparturient hormone fluxes may adversely affect mammary gland immunity and mastitis susceptibility. 17 beta-Estradiol (E-2) and progesterone (P-4) have been reported to function on immune regulation, and their concentration fluctuates dramatically during the perinatal period. Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) mediate numerous aspects of innate immunity in humans and experimental animals. This study aimed to explore the effects of E-2 and P-4 on NOD2 expression in bovine mammary epithelial cells (BMECs). BMECs were isolated and purified from bovine mammary tissue and treated with E-2/P-4 and muramyl dipeptide (MDP). After these treatments, the mRNA levels of NOD2, receptor-interacting protein kinase (RIP) 2, interleukin (IL) 1 beta, IL-6, IL-8 and tumor necrosis factor (TNF) alpha were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) respectively, and the protein levels of NOD2 were analyzed by western blotting. The results showed that E-2 and P-4 decreased MDP-induced transcriptional expression of NOD2 and the downstream molecules. Moreover, E-2 reduced MDP-induced NOD2 protein expression levels. Our study suggests that down-regulation of NOD2 by E-2 and P-4 may be one of the reasons for mastitis susceptibility in periparturient dairy cows.
