In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus
In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus
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DOI：
10.1099/vir.0.81038-0
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发表时间：
2005-09-01
影响因子：
3.8
通讯作者：
Parkhouse, RME
中科院分区：
文献类型：
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作者：
Oura, CAL;Denyer, MS;Parkhouse, RME
为了理解涉及对非洲猪瘟病毒（ASFV）感染的保护性免疫的机制，利用了用无毒力的葡萄牙ASFV分离株OUR/T88/3感染保护远交系猪免受毒力的葡萄牙ASFV分离株OUR/T88/1攻击的观察结果。结果表明，暴露于OUR/T88/3然后耗尽CD 8（+）淋巴细胞的猪不再完全免受OUR/T88/1攻击。这表明CD 8+淋巴细胞在对ASFV感染的保护性免疫应答中起重要作用，并且来自OUR/T88/3感染的抗ASFV抗体单独不足以保护猪免受OUR/T88/1攻击。感染OUR/T88/3的cc单倍型的近交系猪并不总是能免受OUR/T88/1攻击，并出现病毒血症和发热。这种病毒血症总是与循环中CD 8 β（+）淋巴细胞数量增加相关，表明CDB β（+）淋巴细胞在对抗病毒血症中具有特定作用。这些实验表明CD 8+淋巴细胞，特别是CD 8（+）淋巴细胞在ASIF保护性免疫中的重要作用。
To understand the mechanisms involved in protective immunity to African swine fever virus (ASFV) infection, the observation that infection with the avirulent Portuguese ASFV isolate OUR/T88/3 protects outbred pigs from challenge with the virulent Portuguese ASFV isolate OUR/T88/1 was exploited. It was demonstrated that pigs exposed to OUR/T88/3 and then depleted of CD8(+) lymphocytes were no longer fully protected from OUR/T88/1 challenge. This indicated that CD8+ lymphocytes play an important role in the protective immune response to ASFV infection and that anti-ASFV antibody alone, from OUR/T88/3 infection, was not sufficient to protect pigs from OUR/T88/1 challenge. Inbred pigs of the cc haplotype infected with OUR/T88/3 were not always protected from OUR/T88/1 challenge and developed both viraemia and fever. Such viraemia was always correlated with increased numbers of circulating CD8 beta(+) lymphocytes, indicating a specific role for CDB beta(+) lymphocytes in combating viraemia. These experiments indicate an important role for CD8+ lymphocytes, particularly CD8(+) lymphocytes, in ASIF protective immunity.