EFFECTS OF 2-MERCAPTOETHANOL ON SURVIVAL AND DIFFERENTIATION OF FETAL MOUSE-BRAIN NEURONS CULTURED IN-VITRO
EFFECTS OF 2-MERCAPTOETHANOL ON SURVIVAL AND DIFFERENTIATION OF FETAL MOUSE-BRAIN NEURONS CULTURED IN-VITRO
复制标题
DOI：
10.1016/0304-3940(93)90371-q
复制
发表时间：
1993-12-12
影响因子：
2.5
通讯作者：
NAKANISHI, T
中科院分区：
文献类型：
--
作者：
ISHII, K;KATAYAMA, M;NAKANISHI, T
本文研究了2-巯基乙醇对原代培养的胎鼠脑神经元的影响。在培养基中加入2-巯基乙醇分别使胚胎第16天的小鼠纹状体神经元和第18天的大脑皮层神经元的存活率增加6倍或200倍，并且还诱导神经突生长，特别是在皮层神经元中突出。此外，该药物增强VR-2g或BIM细胞的条件培养基的营养活性。这些结果表明，2-巯基乙醇可以支持胎鼠脑神经元的活力和分化。
Effects of 2-mercaptoethanol on primary cultures of fetal mouse brain neurons have been investigated. The addition of 2-mercaptoethanol to the culture medium increased 6- or 200-fold the survival rates of embryonic day-16 murine striatum neurons and day-18 cerebral cortical neurons cultured in serum-free medium, respectively, and also induced neurite outgrowth, particularly being prominent in cortical neurons. Moreover, this drug enhanced trophic activities of the conditioned medium of VR-2g or BIM cells. These findings indicate that 2-mercaptoethanol can support the viability and differentiation of fetal mouse brain neurons.