gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe
gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe
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DOI：
10.1080/09168451.2019.1676695
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发表时间：
2019-10-13
影响因子：
1.6
通讯作者：
Aiba, Hirofumi
中科院分区：
文献类型：
--
作者：
Imai, Yuki;Shimasaki, Takafumi;Aiba, Hirofumi
在利用酵母进行的寿命研究中，按时间顺序的寿命被定义为进入稳定期后的存活时间。以前，筛选粟酒裂殖酵母的长寿突变体，以确定参与长寿的新因素。通过筛选，获得了一株长寿突变体，命名为No.36。在本研究中，我们确定了在编码葡糖基转移酶的gas 1（+）中引起的突变（gas 1 -287突变）负责No.36突变体的寿命。通过对这种突变体的分析，我们发现细胞壁扰动剂米卡芬净也延长了裂变酵母的时间寿命。这种寿命延长依赖于Pmk 1和Sty 1 MAP激酶，并且由gas 1 -287突变引起的寿命也依赖于这些激酶。综上所述，我们认为gas 1 -287突变导致长寿的机制与细胞壁应激相似，依赖于Pmk 1和Sty 1 MAPK通路。
In the longevity research by using yeasts, chronological lifespan is defined as the survival time after entry into stationary phase. Previously, screening for long lived mutants of Schizosaccharomyces pombe was performed to identify the novel factors involved in longevity. From this screening, one long lived mutant called as No.36 was obtained. In this study, we identified the mutation caused in gas1(+), which encodes glucanosyltransferase (gas1-287 mutation) is responsible for the longevity of No.36 mutant. Through the analysis of this mutant, we found that cell wall perturbing agent micafungin also extends chronological lifespan in fission yeast. This lifespan extension depended on both Pmk1 and Sty1 MAP kinases, and longevity caused by the gas1-287 mutation also depended on these kinases. In summary, we propose that the gas1-287 mutation causes longevity as the similar mechanism as cell wall stress depending on Pmk1 and Sty1 MAPK pathways.