Negative regulation of abscisic acid signaling by the Brassica oleracea ABI1 ortholog.
Negative regulation of abscisic acid signaling by the Brassica oleracea ABI1 ortholog.
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DOI：
10.1016/j.bbrc.2013.11.035
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发表时间：
2013-12
影响因子：
3.1
通讯作者：
F. Yuan;Mengyao Wang;H. Hao;Yanfeng Zhang;Huixian Zhao;A. Guo;Hong Xu;Xiaona Zhou;C. Xie
中科院分区：
文献类型：
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作者：
F. Yuan;Mengyao Wang;H. Hao;Yanfeng Zhang;Huixian Zhao;A. Guo;Hong Xu;Xiaona Zhou;C. Xie
ABI1(ABA不敏感1)是早期ABA(脱落酸)信号转导的核心调控网络的重要组成部分。在此，我们研究了甘蓝ABI1同源基因的功能。BolABI1的表达受到干旱的强烈诱导，而BolABI1的结构性表达使ABA对野生型不敏感。亚细胞定位和磷酸酶分析表明，BolABI1主要定位于细胞核，具有磷酸酶活性。此外，BolABI1与OST1(开放气孔1)INB的同源物相互作用。在体外能使ABI5(ABA不敏感5)去磷酸化。总体而言，这些结果表明，BolABI1是一种功能性的PP2C型蛋白磷酸酶，参与了ABA信号通路的负调控。
ABI1 (ABA Insensitive 1) is an important component of the core regulatory network in early ABA (Abscisic acid) signaling. Here, we investigated the functions of an ABI1 ortholog inBrassica oleracea(BolABI1). The expression ofBolABI1was dramatically induced by drought, and constitutive expression ofBolABI1confers ABA insensitivity upon the wild-type. Subcellular localization and phosphatase assays reveal that BolABI1 is predominantly localized in the nucleus and harbors phosphatase activity. Furthermore, BolABI1 interacts with a homolog of OST1 (OPEN STOMATA 1) inB. oleracea(BolOST1) and can dephosphorylate ABI5 (ABA Insensitive 5)in vitro. Overall, these results suggest thatBolABI1is a functional PP2C-type protein phosphatase that is involved in the negative modulation of the ABA signaling pathway.