Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates
Comparison between E-test and CLSI broth microdilution method for antifungal susceptibility testing of Candida albicans oral isolates
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DOI：
10.1590/s0036-46652008000100002
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发表时间：
2008-02-01
期刊：
影响因子：
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通讯作者：
Resende, Maria Aparecida de
中科院分区：
文献类型：
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作者：
Koga-Ito, Cristiane Yumi;Lyon, Juliana Pereira;Resende, Maria Aparecida de
口腔念珠菌病患者分离出30株白色念珠菌，研究从对照个体分离的白色念珠菌。采用CLSI参考方法和E试验系统对红霉素B、氟康唑、5-氟胞嘧啶和伊曲康唑进行体外药敏试验。对所得结果进行了分析和比较。从口腔念珠菌病患者和对照个体分离的菌株的MIC值相似。两种方法的符合率分别为：庆大霉素B 66.67%，氟康唑53.33%，氟胞嘧啶65%，伊曲康唑45%。根据我们的数据，E试验方法由于其简单性，可以完全替代试验常规药敏试验。但是，不能将其视为CLSI参考方法的替代品。
Thirty Candida albicans isolated from oral candidosis patients and 30 C. albicans isolated from control individuals were studied. In vitro susceptibility tests, were performed for amphotericin B, fluconazole, 5-flucytosine and itraconazole through the Clinical and Laboratorial Standards Institute (CLSI) reference method and E test system. The results obtained were analyzed and compared. MIC values were similar for the strains isolated from oral candidosis patients and control individuals. The agreement rate for the two methods was 66.67% for amphotericin B, 53.33% for fluconazole, 65% for flucytosine and 45% for itraconazole. According to Our data, E test method could be all alternative to trial routine susceptibility testing due to its simplicity. However, it can not be considered a substitute for the CLSI reference method.