Serum expression levels of microRNA-382-3p,-598-3p,-1246 and-184 in breast cancer patients
Serum expression levels of microRNA-382-3p,-598-3p,-1246 and-184 in breast cancer patients
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DOI：
10.3892/ol.2016.4582
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发表时间：
2016-07-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Fu, Lun;Li, Zhaoyun;Liu, Yang
本研究的目的是研究乳腺癌患者血清中microRNA (miRNA/miR) -382-3p、-598-3p、-1246和-184的水平，并评估其作为乳腺癌筛查生物标志物的可行性。选取2013年1月至2014年9月在中国浙江省台州市中心医院的100名乳腺癌患者和40名年龄匹配的健康对照者的血清样本。采用茎环逆转录-定量聚合酶链反应检测血清miR-382-3p、-598-3p、-1246和-184的表达水平。绘制受试者工作特征曲线，评价血清miRNA表达水平对筛查乳腺癌的敏感性和特异性。miR-382-3p和-1246在乳腺癌患者血清中显著上调，miR-598-3p和-184在乳腺癌患者血清中显著下调。检测乳腺癌的敏感性和特异性分别为：miR-382-3p、52.0和92.5%；miR-598-3p分别为95.0%和85.0%；miR-1246, 93.0和75.0%；miR-184，分别为87.5%和71.0%。4种血清mirna的表达水平与患者的临床分期无关。综上所述，miR-382-3p, -598-3p， -1246和-184都参与了乳腺癌的发展，是很有希望用于乳腺癌检测的生物标志物。
The purpose of the present study was to investigate the serum levels of microRNA (miRNA/miR) -382-3p, -598-3p, -1246 and -184 in breast cancer patients and to assess their feasibility as biomarkers for breast cancer screening. Serum samples were obtained from 100 breast cancer patients and 40 age-matched healthy control subjects in Taizhou Central Hospital (Taizhou, Zhejiang, China) between January 2013 and September 2014. The serum expression levels of miR-382-3p, -598-3p, -1246 and -184 were determined by stem-loop reverse transcription-quantitative polymerase chain reaction. Receiver operating characteristic curves were drawn to evaluate the sensitivity and specificity of the serum miRNA expression levels for the screening of breast cancer. miR-382-3p and -1246 were significantly upregulated in the serum of the breast cancer patients, while miR-598-3p and -184 were significantly downregulated. The sensitivity and specificity to detect breast cancer were as follows: miR-382-3p, 52.0 and 92.5%; miR-598-3p, 95.0 and 85.0%; miR-1246, 93.0 and 75.0%; and miR-184, 87.5 and 71.0%, respectively. The expression levels of the four serum miRNAs were not correlated with the patients' clinical stage. In summary, miR-382-3p, -598-3p, -1246 and -184 are all involved in the development of breast cancer, and are promising biomarkers for breast cancer detection.