CLONING OF THE COMPLETE HUMAN CYTOMEGALOVIRUS GENOME IN COSMIDS
CLONING OF THE COMPLETE HUMAN CYTOMEGALOVIRUS GENOME IN COSMIDS
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DOI：
10.1016/0378-1119(82)90054-3
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发表时间：
1982-01-01
期刊：
影响因子：
3.5
通讯作者：
COLLINS, J
中科院分区：
文献类型：
--
作者：
FLECKENSTEIN, B;MULLER, I;COLLINS, J
纯化的病毒体DNA（155 ×用限制性内切核酸酶HindIII和EcoRI部分切割人巨细胞病毒（CMV）株Ad169的DNA片段（分子量为106 MW），并将其克隆到粘粒pHC79的相应切割位点。在一组含有病毒DNA的长重叠片段的克隆中建立了完整的基因文库。HindIII（29个片段）和EcoRI（36个片段）的限制性酶切图谱是从粘粒克隆片段的连接，从克隆DNA的双链，以及标记的克隆病毒DNA与病毒体DNA和单或双切割粘粒克隆的限制性酶切片段的印迹杂交中构建的。
Purified virion DNA (155 .times. 106 MW) of human cytomegalovirus (CMV) strain Ad169 was partially cleaved with restriction endonucleases HindIII and EcoRI and cloned in the respective cleavage sites of cosmid pHC79. A complete gene library was established in a set of clones containing the viral DNA in long overlapping segments. Restriction maps for HindIII (29 fragments) and EcoRI (36 fragments) were constructed from the linkage of cosmid-cloned fragments, from double digestions of cloned DNA, and from blot hybridizations of labeled cloned viral DNA with restriction fragments of virion DNA and singly or doubly cleaved cosmid clones.